Construct: ORF ccsbBroad304_12692

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005176.1_s304c1, BRDN0000404548
Derived from:
ccsbBroadEn_12692
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CPEB4 (80315)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12692
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80315 CPEB4 cytoplasmic polyadenylation... NM_001308193.1 100% 100%
2 human 80315 CPEB4 cytoplasmic polyadenylation... NM_001308192.1 94.9% 94.9% 61_111del
3 human 132864 CPEB2 cytoplasmic polyadenylation... XM_017007733.1 54.8% 63% (many diffs)
4 human 132864 CPEB2 cytoplasmic polyadenylation... XM_017007734.2 54.8% 63% (many diffs)
5 human 80315 CPEB4 cytoplasmic polyadenylation... NM_001308191.2 45.7% 45.7% 1_1146del
6 human 80315 CPEB4 cytoplasmic polyadenylation... NM_001308189.2 45.2% 45.2% 1_1146del;1206_1229del
7 human 80315 CPEB4 cytoplasmic polyadenylation... XM_005265994.1 44.6% 44.6% 1_1146del;1207_1257del
8 human 80315 CPEB4 cytoplasmic polyadenylation... NM_030627.4 44.1% 44.1% 1_1146del;1206_1280del
9 human 22849 CPEB3 cytoplasmic polyadenylation... XM_011539515.2 37.1% 41.4% (many diffs)
10 human 132864 CPEB2 cytoplasmic polyadenylation... NM_182646.3 25.6% 29.3% (many diffs)
11 human 132864 CPEB2 cytoplasmic polyadenylation... NM_001177384.2 25.6% 29.2% (many diffs)
12 human 132864 CPEB2 cytoplasmic polyadenylation... NM_001177383.2 25.4% 29.1% (many diffs)
13 human 132864 CPEB2 cytoplasmic polyadenylation... NM_001177381.2 25.3% 29% (many diffs)
14 human 132864 CPEB2 cytoplasmic polyadenylation... NM_182485.3 24.9% 28.5% (many diffs)
15 human 132864 CPEB2 cytoplasmic polyadenylation... XM_011513777.3 24.8% 28.4% (many diffs)
16 human 132864 CPEB2 cytoplasmic polyadenylation... NM_001177382.2 24.7% 28.2% (many diffs)
17 human 132864 CPEB2 cytoplasmic polyadenylation... XM_005248135.3 24.6% 28.2% (many diffs)
18 mouse 67579 Cpeb4 cytoplasmic polyadenylation... NM_001290678.1 42.9% 45.4% (many diffs)
19 mouse 67579 Cpeb4 cytoplasmic polyadenylation... XM_006514793.1 42.5% 44.9% (many diffs)
20 mouse 67579 Cpeb4 cytoplasmic polyadenylation... NM_001290676.1 41.9% 44.3% (many diffs)
21 mouse 67579 Cpeb4 cytoplasmic polyadenylation... NM_026252.4 41.5% 43.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1032
ORF length:
966
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca ctcactggag agttcactca ttgacataat gagagctgaa aatgatacca
121ttaaaggtca gtcttcactg tttccaatgg aagatggatt cttggatgat ggccgtgggg
181atcagcctct tcatagtggc ctgggttcac ctcactgctt cagtcaccag aatggggaaa
241gagtggaacg atattctcga aaggtgtttg taggcggatt gcctccagac attgatgaag
301atgagatcac agctagtttt cgtcgctttg gccctctgat tgtggattgg cctcataaag
361ctgagagcaa atcctatttt cctcctaaag gctatgcatt cctgctgttt caagatgaaa
421gctctgtgca ggctctcatt gatgcatgca ttgaagaaga tggaaaactc tacctttgtg
481tatcaagtcc cactatcaag gataagccag tccagattcg gccttggaat ctcagtgaca
541gtgactttgt gatggatggt tcacagccac ttgacccacg aaaaactata tttgttggtg
601gtgttcctcg accattacga gctgtggagc ttgcgatgat aatggatcgg ctatatggag
661gtgtgtgcta cgctgggatt gataccgacc ctgagctaaa atacccaaaa ggagctggga
721gagttgcgtt ctctaatcaa cagagttaca tagctgctat cagtgcccgc tttgttcagc
781tgcagcatgg agagatagat aaacgggtgg aagttaagcc atatgtcttg gatgatcagc
841tgtgtgatga atgtcagggg gcccgttgtg gggggaaatt tgctccattt ttctgtgcta
901atgttacctg tctgcagtat tactgtgaat attgctgggc tgctatccat tctcgtgctg
961gcagggaatt ccacaagccc ctggtgaagg aaggcggtga ccgccctcgg catatttcat
1021tccgctggaa ctacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1081tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1141caatcaacct ctggatta

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