Transcript: Mouse NM_133995.4

Mus musculus ureidopropionase, beta (Upb1), mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Upb1 (103149)
Length:
3042
CDS:
146..1327

Additional Resources:

NCBI RefSeq record:
NM_133995.4
NBCI Gene record:
Upb1 (103149)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133995.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000101747 GATCAATGATTTCTGGACTTT pLKO.1 1192 CDS 100% 4.950 3.960 N Upb1 n/a
2 TRCN0000101748 GTGGAGTCAATATCATCTGTT pLKO.1 474 CDS 100% 4.950 3.960 N Upb1 n/a
3 TRCN0000101745 GAGGGTAGAATAGGCACTTAA pLKO.1 1405 3UTR 100% 13.200 9.240 N Upb1 n/a
4 TRCN0000101746 GCAGGATTGCAGTGAACATTT pLKO.1 822 CDS 100% 13.200 9.240 N Upb1 n/a
5 TRCN0000101749 CAATGATTTCTGGACTTTCAA pLKO.1 1195 CDS 100% 5.625 3.938 N Upb1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133995.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08341 pDONR223 100% 81.9% 83.4% None (many diffs) n/a
2 ccsbBroad304_08341 pLX_304 0% 81.9% 83.4% V5 (many diffs) n/a
3 TRCN0000475558 CTTCCGAGTGTGCTTCCCCACTAG pLX_317 26.7% 81.9% 83.4% V5 (many diffs) n/a
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