Transcript: Human NM_134261.3

Homo sapiens RAR related orphan receptor A (RORA), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
RORA (6095)
Length:
10827
CDS:
85..1656

Additional Resources:

NCBI RefSeq record:
NM_134261.3
NBCI Gene record:
RORA (6095)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_134261.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000022155 CCAAACGCATTGATGGATTTA pLKO.1 1097 CDS 100% 13.200 18.480 N RORA n/a
2 TRCN0000022157 GAATCCATTATGGTGTCATTA pLKO.1 329 CDS 100% 13.200 18.480 N RORA n/a
3 TRCN0000437977 ATAGCGGAGGTTGCGGCATTA pLKO_005 2052 3UTR 100% 10.800 15.120 N RORA n/a
4 TRCN0000022154 CCAGACATTGTGCGACTTCAT pLKO.1 1570 CDS 100% 4.950 6.930 N RORA n/a
5 TRCN0000431023 ATGCAAATTGATGGGTAAATG pLKO_005 1639 CDS 100% 13.200 9.240 N RORA n/a
6 TRCN0000430461 CAACAGGAGGAGGGTACTAAA pLKO_005 1924 3UTR 100% 13.200 9.240 N RORA n/a
7 TRCN0000022158 CCTTAGGTTGTGAAGACTTTA pLKO.1 1262 CDS 100% 13.200 9.240 N RORA n/a
8 TRCN0000022156 GCATCTGGAAACCTGCCAATA pLKO.1 933 CDS 100% 10.800 7.560 N RORA n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_134261.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488554 CCGGAAAGGCAAATATTAGTTTAT pLX_317 22.8% 99.9% 100% V5 (not translated due to prior stop codon) 1428C>A n/a
2 TRCN0000491974 CACAAGTCCTCGTCATGAGGGAAC pLX_317 17.3% 88% 87.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_13944 pDONR223 100% 88% 86.9% None (many diffs) n/a
4 ccsbBroad304_13944 pLX_304 0% 88% 86.9% V5 (not translated due to frame shift) (many diffs) n/a
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