Construct: ORF TRCN0000488554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021769.1_s317c1
- DNA Barcode:
- CCGGAAAGGCAAATATTAGTTTAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RORA (6095)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6095 | RORA | RAR related orphan receptor A | NM_134261.3 | 99.9% | 100% | 1428C>A |
| 2 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521875.2 | 92.5% | 89.3% | (many diffs) |
| 3 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521874.1 | 90.5% | 86.5% | (many diffs) |
| 4 | human | 6095 | RORA | RAR related orphan receptor A | XM_005254584.5 | 90.3% | 89.6% | (many diffs) |
| 5 | human | 6095 | RORA | RAR related orphan receptor A | NM_002943.3 | 88.7% | 84.5% | (many diffs) |
| 6 | human | 6095 | RORA | RAR related orphan receptor A | NM_134262.3 | 88% | 87.3% | (many diffs) |
| 7 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521877.3 | 87.6% | 87.5% | (many diffs) |
| 8 | human | 6095 | RORA | RAR related orphan receptor A | XM_017022466.2 | 87.6% | 87.3% | (many diffs) |
| 9 | human | 6095 | RORA | RAR related orphan receptor A | NM_134260.2 | 87.2% | 83.1% | (many diffs) |
| 10 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521878.2 | 73.7% | 73.8% | 0_1ins411;1017C>A |
| 11 | human | 6095 | RORA | RAR related orphan receptor A | XM_017022467.2 | 73.7% | 73.8% | 0_1ins411;1017C>A |
| 12 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521879.3 | 71.8% | 71.8% | 0_1ins441;987C>A |
| 13 | mouse | 19883 | Rora | RAR-related orphan receptor... | NM_013646.2 | 91% | 97.3% | (many diffs) |
| 14 | mouse | 19883 | Rora | RAR-related orphan receptor... | XM_006510877.3 | 84.8% | 87.8% | (many diffs) |
| 15 | mouse | 19883 | Rora | RAR-related orphan receptor... | XM_006510878.2 | 81.9% | 87.1% | (many diffs) |
| 16 | mouse | 19883 | Rora | RAR-related orphan receptor... | NM_001289916.1 | 80.5% | 86.9% | (many diffs) |
| 17 | mouse | 19883 | Rora | RAR-related orphan receptor... | XM_006510880.1 | 80% | 86.9% | (many diffs) |
| 18 | mouse | 19883 | Rora | RAR-related orphan receptor... | XM_017313219.1 | 67% | 73.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1638
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagtcagct ccggcagccc ccgaccccgc cgccagcgag ccaggcagca 121 gcggcgcgga cgcggccgcc ggctccaggg agaccccgct gaaccaggaa tccgcccgca 181 agagcgagcc gcctgccccg gtgcgcagac agagctattc cagcaccagc agaggtatct 241 cagtaacgaa gaagacacat acatctcaaa ttgaaattat tccatgcaag atctgtggag 301 acaaatcatc aggaatccat tatggtgtca ttacatgtga aggctgcaag ggctttttca 361 ggagaagtca gcaaagcaat gccacctact cctgtcctcg tcagaagaac tgtttgattg 421 atcgaaccag tagaaaccgc tgccaacact gtcgattaca gaaatgcctt gccgtaggga 481 tgtctcgaga tgctgtaaaa tttggccgaa tgtcaaaaaa gcagagagac agcttgtatg 541 cagaagtaca gaaacaccgg atgcagcagc agcagcgcga ccaccagcag cagcctggag 601 aggctgagcc gctgacgccc acctacaaca tctcggccaa cgggctgacg gaacttcacg 661 acgacctcag taactacatt gacgggcaca cccctgaggg gagtaaggca gactccgccg 721 tcagcagctt ctacctggac atacagcctt ccccagacca gtcaggtctt gatatcaatg 781 gaatcaaacc agaaccaata tgtgactaca caccagcatc aggcttcttt ccctactgtt 841 cgttcaccaa cggcgagact tccccaactg tgtccatggc agaattagaa caccttgcac 901 agaatatatc taaatcgcat ctggaaacct gccaatactt gagagaagag ctccagcaga 961 taacgtggca gaccttttta caggaagaaa ttgagaacta tcaaaacaag cagcgggagg 1021 tgatgtggca attgtgtgcc atcaaaatta cagaagctat acagtatgtg gtggagtttg 1081 ccaaacgcat tgatggattt atggaactgt gtcaaaatga tcaaattgtg cttctaaaag 1141 caggttctct agaggtggtg tttatcagaa tgtgccgtgc ctttgactct cagaacaaca 1201 ccgtgtactt tgatgggaag tatgccagcc ccgacgtctt caaatcctta ggttgtgaag 1261 actttattag ctttgtgttt gaatttGGAA AGAGTTTATG TTCTATGCAC CTGACTGAAG 1321 ATGAAATTGC ATTATTTTCT GCATTTGTAC TGATGTCAGC AGATCGCTCA TGGCTGCAAG 1381 AAAAGGTAAA AATTGAAAAA CTGCAACAGA AAATTCAGCT AGCTCTTCAA CACGTCCTAC 1441 AGAAGAATCA CCGAGAAGAT GGAATACTAA CAAAGTTAAT ATGCAAGGTG TCTACATTAA 1501 GAGCCTTATG TGGACGACAT ACAGAAAAGC TAATGGCATT TAAAGCAATA TACCCAGACA 1561 TTGTGCGACT TCATTTTCCT CCATTATACA AGGAGTTGTT CACTTCAGAA TTTGAGCCAG 1621 CAATGCAAAT TGATGGGTAA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGG AAAGGCAAAT 1801 ATTAGTTTAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt