Construct: ORF TRCN0000491974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021549.2_s317c1
- DNA Barcode:
- CACAAGTCCTCGTCATGAGGGAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RORA (6095)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6095 | RORA | RAR related orphan receptor A | NM_134262.3 | 99.9% | 100% | 1263C>A |
| 2 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521877.3 | 98.7% | 98.2% | (many diffs) |
| 3 | human | 6095 | RORA | RAR related orphan receptor A | XM_017022466.2 | 98.1% | 98% | 0_1ins24;6T>A;1239C>A |
| 4 | human | 6095 | RORA | RAR related orphan receptor A | XM_005254584.5 | 97.5% | 96.4% | (many diffs) |
| 5 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521875.2 | 92.3% | 90.6% | (many diffs) |
| 6 | human | 6095 | RORA | RAR related orphan receptor A | NM_134261.3 | 88% | 87.3% | (many diffs) |
| 7 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521874.1 | 87.1% | 87.4% | (many diffs) |
| 8 | human | 6095 | RORA | RAR related orphan receptor A | NM_002943.3 | 84.9% | 83.9% | (many diffs) |
| 9 | human | 6095 | RORA | RAR related orphan receptor A | NM_134260.2 | 82.8% | 81.9% | (many diffs) |
| 10 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521878.2 | 82.4% | 82.4% | 0_1ins246;1017C>A |
| 11 | human | 6095 | RORA | RAR related orphan receptor A | XM_017022467.2 | 82.4% | 82.4% | 0_1ins246;1017C>A |
| 12 | human | 6095 | RORA | RAR related orphan receptor A | XM_011521879.3 | 80.2% | 80.3% | 0_1ins276;987C>A |
| 13 | mouse | 19883 | Rora | RAR-related orphan receptor... | NM_001289916.1 | 91.3% | 99.3% | (many diffs) |
| 14 | mouse | 19883 | Rora | RAR-related orphan receptor... | XM_006510880.1 | 89.6% | 97.4% | (many diffs) |
| 15 | mouse | 19883 | Rora | RAR-related orphan receptor... | XM_006510878.2 | 88.5% | 96.2% | (many diffs) |
| 16 | mouse | 19883 | Rora | RAR-related orphan receptor... | XM_006510877.3 | 81.9% | 87.8% | (many diffs) |
| 17 | mouse | 19883 | Rora | RAR-related orphan receptor... | NM_013646.2 | 80.7% | 86.9% | (many diffs) |
| 18 | mouse | 19883 | Rora | RAR-related orphan receptor... | XM_017313219.1 | 74.9% | 82% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1476
- ORF length:
- 1404
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgatgtat tttgtgatcg cagcgatgaa agctcaaatt gaaattattc 121 catgcaagat ctgtggagac aaatcatcag gaatccatta tggtgtcatt acatgtgaag 181 gctgcaaggg ctttttcagg agaagtcagc aaagcaatgc cacctactcc tgtcctcgtc 241 agaagaactg tttgattgat cgaaccagta gaaaccgctg ccaacactgt cgattacaga 301 aatgccttgc cgtagggatg tctcgagatg ctgtaaaatt tggccgaatg tcaaaaaagc 361 agagagacag cttgtatgca gaagtacaga aacaccggat gcagcagcag cagcgcgacc 421 accagcagca gcctggagag gctgagccgc tgacgcccac ctacaacatc tcggccaacg 481 ggctgacgga acttcacgac gacctcagta actacattga cgggcacacc cctgagggga 541 gtaaggcaga ctccgccgtc agcagcttct acctggacat acagccttcc ccagaccagt 601 caggtcttga tatcaatgga atcaaaccag aaccaatatg tgactacaca ccagcatcag 661 gcttctttcc ctactgttcg ttcaccaacg gcgagacttc cccaactgtg tccatggcag 721 aattagaaca ccttgcacag aatatatcta aatcgcatct ggaaacctgc caatacttga 781 gagaagagct ccagcagata acgtggcaga cctttttaca ggaagaaatt gagaactatc 841 aaaacaagca gcgggaggtg atgtggcaat tgtgtgccat caaaattaca gaagctatac 901 agtatgtggt ggagtttgcc aaacgcattg atggatttat ggaactgtgt caaaatgatc 961 aaattgtgct tctaaaagca ggttctctag aggtggtgtt tatcagaatg tgccgtgcct 1021 ttgactctca gaacaacacc gtgtactttg atgggaagta tgccagcccc gacgtcttca 1081 aatccttagg ttgtgaagac tttattagct ttgtgtttga atttggaaag agtttatgtt 1141 ctatgcacct gactgaagat gaaattgcat tattttctgc atttgtactg atgtcagcag 1201 atcgctcatg gctgcaagaa aaggtaaaaa ttgaaaaact gCAACAGAAA ATTCAGCTAG 1261 CTCTTCAACA CGTCCTACAG AAGAATCACC GAGAAGATGG AATACTAACA AAGTTAATAT 1321 GCAAGGTGTC TACATTAAGA GCCTTATGTG GACGACATAC AGAAAAGCTA ATGGCATTTA 1381 AAGCAATATA CCCAGACATT GTGCGACTTC ATTTTCCTCC ATTATACAAG GAGTTGTTCA 1441 CTTCAGAATT TGAGCCAGCA ATGCAAATTG ATGGGTAGAA CCCAGCTTTC TTGTACAAAG 1501 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1561 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1621 ACGACACAAG TCCTCGTCAT GAGGGAACAC GCGTTAAGTC gacaatcaac ctctggatta 1681 caaaatttgt gaaagatt