Transcript: Mouse NM_181348.4

Mus musculus prune homolog 2 (Prune2), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Prune2 (353211)
Length:
12512
CDS:
140..9394

Additional Resources:

NCBI RefSeq record:
NM_181348.4
NBCI Gene record:
Prune2 (353211)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_181348.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000234256 GAACAGGTCCACGCAGTTATT pLKO_005 194 CDS 100% 13.200 9.240 N Prune2 n/a
2 TRCN0000218681 ACTTCACAGAGACAAGGTTTA pLKO_005 342 CDS 100% 10.800 7.560 N Prune2 n/a
3 TRCN0000218281 ATGAAGGGAAACTGTCGATAA pLKO_005 435 CDS 100% 10.800 7.560 N Prune2 n/a
4 TRCN0000234257 ACAATGCTGAAAGATCTAAAG pLKO_005 815 CDS 100% 10.800 6.480 N Prune2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_181348.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13308 pDONR223 100% 5.4% 5.8% None (many diffs) n/a
2 ccsbBroad304_13308 pLX_304 0% 5.4% 5.8% V5 (many diffs) n/a
3 TRCN0000471170 ATCTCCTTCTAATACTGGGGTTTC pLX_317 77.5% 5.4% 5.8% V5 (many diffs) n/a
Download CSV