Construct: ORF ccsbBroadEn_13308

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018451.1_s300c1, BRDN0000393200
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRUNE2 (158471)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308051.1 56.4% 57% (many diffs)
2 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014359.2 54.5% 55% (many diffs)
3 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308049.1 53.8% 54.4% (many diffs)
4 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014358.2 53.8% 54.4% (many diffs)
5 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014357.2 53.8% 54.4% (many diffs)
6 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014356.2 52% 52.5% (many diffs)
7 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001330680.1 51.9% 52.4% (many diffs)
8 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308050.1 51.6% 52.1% (many diffs)
9 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014354.2 50.2% 50.6% (many diffs)
10 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746212.2 12.5% (many diffs)
11 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251754.2 6% 6% (many diffs)
12 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308048.1 6% 6% (many diffs)
13 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014353.2 6% 6% (many diffs)
14 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518328.2 6% 6% (many diffs)
15 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014351.1 6% 6% (many diffs)
16 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251751.1 6% 6% (many diffs)
17 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014348.1 6% 6% (many diffs)
18 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518327.1 6% 6% (many diffs)
19 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308047.1 6% 6% (many diffs)
20 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518326.2 6% 5.9% (many diffs)
21 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251750.1 6% 5.9% (many diffs)
22 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014352.1 6% 5.9% (many diffs)
23 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518323.2 6% 5.9% (many diffs)
24 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251746.1 5.9% 5.9% (many diffs)
25 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014350.1 5.9% 5.9% (many diffs)
26 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251745.1 5.9% 5.9% (many diffs)
27 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014349.1 5.9% 5.9% (many diffs)
28 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014347.2 5.9% 5.9% (many diffs)
29 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716984.2 5.9% 5.9% (many diffs)
30 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716985.1 5.9% 5.9% (many diffs)
31 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716982.1 5.9% 5.9% (many diffs)
32 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014346.1 5.9% 5.9% (many diffs)
33 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_015225.3 5.9% 5.9% (many diffs)
34 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251748.1 5.9% 5.9% (many diffs)
35 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014345.2 5.9% 5.9% (many diffs)
36 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716986.1 5.9% 5.9% (many diffs)
37 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716983.1 5.9% 5.9% (many diffs)
38 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746211.2 4.3% (many diffs)
39 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746210.2 4.3% (many diffs)
40 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746209.2 4.3% (many diffs)
41 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_428517.3 4.3% (many diffs)
42 human 158471 PRUNE2 prune homolog 2 with BCH do... NR_131751.1 4.1% (many diffs)
43 mouse 353211 Prune2 prune homolog 2 XM_006527154.2 5.5% 5.9% (many diffs)
44 mouse 353211 Prune2 prune homolog 2 XM_006527153.2 5.5% 5.9% (many diffs)
45 mouse 353211 Prune2 prune homolog 2 XM_017318230.1 5.5% 5.8% (many diffs)
46 mouse 353211 Prune2 prune homolog 2 XM_006527152.2 5.5% 5.8% (many diffs)
47 mouse 353211 Prune2 prune homolog 2 XM_006527151.2 5.5% 5.8% (many diffs)
48 mouse 353211 Prune2 prune homolog 2 XM_006527149.2 5.4% 5.8% (many diffs)
49 mouse 353211 Prune2 prune homolog 2 XM_017318228.1 5.4% 5.8% (many diffs)
50 mouse 353211 Prune2 prune homolog 2 XM_017318227.1 5.4% 5.8% (many diffs)
51 mouse 353211 Prune2 prune homolog 2 XM_006527147.2 5.4% 5.8% (many diffs)
52 mouse 353211 Prune2 prune homolog 2 XM_006527146.1 5.4% 5.8% (many diffs)
53 mouse 353211 Prune2 prune homolog 2 NM_181348.4 5.4% 5.8% (many diffs)
54 mouse 353211 Prune2 prune homolog 2 XM_006527144.2 5.4% 5.8% (many diffs)
55 mouse 353211 Prune2 prune homolog 2 XM_017318229.1 5.4% 5.8% (many diffs)
56 mouse 353211 Prune2 prune homolog 2 XM_017318226.1 5.4% 5.8% (many diffs)
57 mouse 353211 Prune2 prune homolog 2 XM_006527145.1 5.4% 5.7% (many diffs)
58 mouse 353211 Prune2 prune homolog 2 XR_386485.2 3.5% (many diffs)
59 mouse 353211 Prune2 prune homolog 2 XR_386484.2 3.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
639
ORF length:
573
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA CATCCCCTTT GAAGAGGGCG TGCTGAGTCC CAGTGCTGCA GACATGAGGC
121CTGAACCTCC TAATTCTCTG GATCTTAATG ACACTCATCC TCGGAGAATC AAGCTCACAG
181CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT
241TGGACAGTCC TGATGAAATT GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG
301ATTCTTTTGA GTACACTGGC CATGAAGATC CCACAGCCAA CAAAGATTCT GGCCAAGAGT
361CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG CTTTGGAGGA
421CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG CCCTACAGGA
481GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT GTGTTTGCCG
541CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA AATCTTTTCC
601TATATGTAAT AAGTACTTTC ACACTACAGC CGCAGAGCTG CCCAACTTTC TTGTACAAAG
661Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)