Construct: ORF TRCN0000471170

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018451.1_s317c1
Derived from:
ccsbBroadEn_13308
DNA Barcode:
ATCTCCTTCTAATACTGGGGTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRUNE2 (158471)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471170
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308051.1 56.4% 57% (many diffs)
2 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014359.2 54.5% 55% (many diffs)
3 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308049.1 53.8% 54.4% (many diffs)
4 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014358.2 53.8% 54.4% (many diffs)
5 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014357.2 53.8% 54.4% (many diffs)
6 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014356.2 52% 52.5% (many diffs)
7 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001330680.1 51.9% 52.4% (many diffs)
8 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308050.1 51.6% 52.1% (many diffs)
9 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014354.2 50.2% 50.6% (many diffs)
10 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746212.2 12.5% (many diffs)
11 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251754.2 6% 6% (many diffs)
12 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308048.1 6% 6% (many diffs)
13 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014353.2 6% 6% (many diffs)
14 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518328.2 6% 6% (many diffs)
15 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014351.1 6% 6% (many diffs)
16 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251751.1 6% 6% (many diffs)
17 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014348.1 6% 6% (many diffs)
18 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518327.1 6% 6% (many diffs)
19 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_001308047.1 6% 6% (many diffs)
20 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518326.2 6% 5.9% (many diffs)
21 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251750.1 6% 5.9% (many diffs)
22 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014352.1 6% 5.9% (many diffs)
23 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_011518323.2 6% 5.9% (many diffs)
24 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251746.1 5.9% 5.9% (many diffs)
25 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014350.1 5.9% 5.9% (many diffs)
26 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251745.1 5.9% 5.9% (many diffs)
27 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014349.1 5.9% 5.9% (many diffs)
28 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014347.2 5.9% 5.9% (many diffs)
29 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716984.2 5.9% 5.9% (many diffs)
30 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716985.1 5.9% 5.9% (many diffs)
31 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716982.1 5.9% 5.9% (many diffs)
32 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014346.1 5.9% 5.9% (many diffs)
33 human 158471 PRUNE2 prune homolog 2 with BCH do... NM_015225.3 5.9% 5.9% (many diffs)
34 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_005251748.1 5.9% 5.9% (many diffs)
35 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_017014345.2 5.9% 5.9% (many diffs)
36 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716986.1 5.9% 5.9% (many diffs)
37 human 158471 PRUNE2 prune homolog 2 with BCH do... XM_006716983.1 5.9% 5.9% (many diffs)
38 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746211.2 4.3% (many diffs)
39 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746210.2 4.3% (many diffs)
40 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_001746209.2 4.3% (many diffs)
41 human 158471 PRUNE2 prune homolog 2 with BCH do... XR_428517.3 4.3% (many diffs)
42 human 158471 PRUNE2 prune homolog 2 with BCH do... NR_131751.1 4.1% (many diffs)
43 mouse 353211 Prune2 prune homolog 2 XM_006527154.2 5.5% 5.9% (many diffs)
44 mouse 353211 Prune2 prune homolog 2 XM_006527153.2 5.5% 5.9% (many diffs)
45 mouse 353211 Prune2 prune homolog 2 XM_017318230.1 5.5% 5.8% (many diffs)
46 mouse 353211 Prune2 prune homolog 2 XM_006527152.2 5.5% 5.8% (many diffs)
47 mouse 353211 Prune2 prune homolog 2 XM_006527151.2 5.5% 5.8% (many diffs)
48 mouse 353211 Prune2 prune homolog 2 XM_006527149.2 5.4% 5.8% (many diffs)
49 mouse 353211 Prune2 prune homolog 2 XM_017318228.1 5.4% 5.8% (many diffs)
50 mouse 353211 Prune2 prune homolog 2 XM_017318227.1 5.4% 5.8% (many diffs)
51 mouse 353211 Prune2 prune homolog 2 XM_006527147.2 5.4% 5.8% (many diffs)
52 mouse 353211 Prune2 prune homolog 2 XM_006527146.1 5.4% 5.8% (many diffs)
53 mouse 353211 Prune2 prune homolog 2 NM_181348.4 5.4% 5.8% (many diffs)
54 mouse 353211 Prune2 prune homolog 2 XM_006527144.2 5.4% 5.8% (many diffs)
55 mouse 353211 Prune2 prune homolog 2 XM_017318229.1 5.4% 5.8% (many diffs)
56 mouse 353211 Prune2 prune homolog 2 XM_017318226.1 5.4% 5.8% (many diffs)
57 mouse 353211 Prune2 prune homolog 2 XM_006527145.1 5.4% 5.7% (many diffs)
58 mouse 353211 Prune2 prune homolog 2 XR_386485.2 3.5% (many diffs)
59 mouse 353211 Prune2 prune homolog 2 XR_386484.2 3.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
639
ORF length:
573
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga catccccttt gaagagggcg tgctgagtcc cagtgctgca gacatgaggc
121ctgaacctcc taattctctg gatcttaatg acactcatcc tcggagaatc aagctcacag
181ccccaaataT CAATCTTTCT CTGGACCAAA GTGAAGGATC TATTCTCTCT GATGATAACT
241TGGACAGTCC TGATGAAATT GACATCAATG TGGATGAACT TGATACCCCC GATGAAGCAG
301ATTCTTTTGA GTACACTGGC CATGAAGATC CCACAGCCAA CAAAGATTCT GGCCAAGAGT
361CAGAGTCTAT TCCAGAATAT ACGGCCGAAG AGGAACGGGA GGACAACCGG CTTTGGAGGA
421CAGTGGTCAT TGGAGAACAA GAGCAGCGCA TTGACATGAA GGTCATCGAG CCCTACAGGA
481GAGTCATTTC TCACGGAGGA TACTATGGGG ACGGTCTAAA TGCCATCATT GTGTTTGCCG
541CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA AATCTTTTCC
601TATATGTAAT AAGTACTTTC ACACTACAGC CGCAGAGCTG CCCAACTTTC TTGTACAAAG
661TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
721GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
781ACGAATCTCC TTCTAATACT GGGGTTTCAC GCGTTAAGTC gacaatcaac ctctggatta
841caaaatttgt gaaagatt

Download FASTA (ORF) (Full)