Transcript: Mouse NM_211357.2

Mus musculus EYA transcriptional coactivator and phosphatase 3 (Eya3), transcript variant 3, mRNA.

Source:
NCBI, updated 2017-06-18
Taxon:
Mus musculus (mouse)
Gene:
Eya3 (14050)
Length:
4990
CDS:
323..1573

Additional Resources:

NCBI RefSeq record:
NM_211357.2
NBCI Gene record:
Eya3 (14050)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_211357.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000029858 CCCTCGCTCATCCAATGATTA pLKO.1 309 5UTR 100% 13.200 18.480 N Eya3 n/a
2 TRCN0000231182 CCCTCGCTCATCCAATGATTA pLKO_005 309 5UTR 100% 13.200 18.480 N Eya3 n/a
3 TRCN0000231183 GATTATCCCACCTATACTATT pLKO_005 440 CDS 100% 13.200 18.480 N Eya3 n/a
4 TRCN0000231184 AGTGAATTGGAACGGGTATTT pLKO_005 749 CDS 100% 13.200 10.560 N Eya3 n/a
5 TRCN0000231181 ACATCCAGCCTTGCGTCAAAT pLKO_005 247 5UTR 100% 13.200 9.240 N Eya3 n/a
6 TRCN0000029854 CGTGTAAGAAACACTCATAAA pLKO.1 3912 3UTR 100% 13.200 9.240 N Eya3 n/a
7 TRCN0000231185 CTGGACAACGCCACCTATTAG pLKO_005 3408 3UTR 100% 13.200 9.240 N Eya3 n/a
8 TRCN0000029855 CCAATGATTATACCTCACAAA pLKO.1 320 5UTR 100% 4.950 3.465 N Eya3 n/a
9 TRCN0000029856 CCACAACATTAGCAGCTACAA pLKO.1 540 CDS 100% 4.950 3.465 N Eya3 n/a
10 TRCN0000029857 CCAGGCTTTAGAGCTTGACTT pLKO.1 1546 CDS 100% 4.950 3.465 N Eya3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_211357.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491915 CCGTCATCTACTAAGTACAGCCCC pLX_317 23.9% 68.6% 65.8% V5 (many diffs) n/a
2 ccsbBroadEn_10815 pDONR223 100% 68.5% 65.8% None (many diffs) n/a
3 ccsbBroad304_10815 pLX_304 0% 68.5% 65.8% V5 (many diffs) n/a
4 TRCN0000467722 GACCTGGTTTTCTGTAAATTCGCG pLX_317 23.4% 68.5% 65.8% V5 (many diffs) n/a
5 TRCN0000489799 TACGGGGGAAATTGGTGCTGCGTT pLX_317 22.1% 68.5% 65.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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