Construct: ORF ccsbBroad304_10815
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003999.1_s304c1, BRDN0000407697
- Derived from:
- ccsbBroadEn_10815
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EYA3 (2140)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10815
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NM_001282560.2 | 100% | 100% | |
| 2 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_011541003.2 | 99.6% | 99.6% | 34_39delAGAAAG |
| 3 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NM_001282561.1 | 96.2% | 90.4% | (many diffs) |
| 4 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_011541004.2 | 95.9% | 90.1% | (many diffs) |
| 5 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_011541001.3 | 92% | 92% | 361_498del |
| 6 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_024453995.1 | 92% | 92% | 361_498del |
| 7 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_011540999.2 | 91.7% | 91.7% | 34_39delAGAAAG;367_504del |
| 8 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_024453987.1 | 91.7% | 91.7% | 34_39delAGAAAG;367_504del |
| 9 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_024454014.1 | 90.1% | 90.1% | 0_1ins159 |
| 10 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NM_001990.4 | 88.7% | 83.5% | (many diffs) |
| 11 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_006710449.3 | 88.4% | 83.3% | (many diffs) |
| 12 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_024454013.1 | 82.9% | 82.9% | 0_1ins159;202_339del |
| 13 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NM_001282562.1 | 79.6% | 74.7% | (many diffs) |
| 14 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NR_104214.1 | 73.3% | (many diffs) | |
| 15 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XR_001737039.2 | 63.5% | 1_158del;1102_1169del;1391_1392ins443 | |
| 16 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XR_001737038.1 | 25.6% | (many diffs) | |
| 17 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | NM_210071.2 | 86.7% | 85.6% | (many diffs) |
| 18 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | NM_010166.3 | 83.9% | 82.7% | (many diffs) |
| 19 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | XM_006538537.3 | 79.9% | 79.1% | (many diffs) |
| 20 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | XM_006538538.3 | 77.4% | 76.4% | (many diffs) |
| 21 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | NM_211357.2 | 68.5% | 65.8% | (many diffs) |
| 22 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | XM_006538540.3 | 49.3% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1674
- ORF length:
- 1608
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agaagagcaa gatttaccag agcaaccagt gaaaaaagcc aagatgcagg 121 aatcaggaga gcaaactata agtcaagtaa gcaatccaga tgtcagtgat cagaagcctg 181 aaacatcaag ccttgcttca aaccttccca tgtcagagga aattatgaca tgcaccgatt 241 acatccctcg ctcatccaat gattatacct cacaaatgta ttctgcaaaa ccttatgcac 301 atattctctc agttcctgtt tcggaaactg cttaccctgg acagactcaa taccagacac 361 tacagcagac tcaaccctat gctgtctacc ctcaggcaac ccaaacgtat ggactacctc 421 cttttgcttc aagcacaaat gccagcctga tatctacttc ttctacaatt gccaatattc 481 cagcagcagc agtagccagc atctcaaacc aggattatcc cacctatact attcttggtc 541 agaatcagta ccaggcctgc taccccagct ccagctttgg agtcacaggt cagactaaca 601 gtgatgcaga gagcaccaca ttagcagcaa ccacatacca gtcggagaag cctagtgtca 661 tggcgcctgc acctgcagca cagagacttt cctctggaga cccttctaca agtccatctt 721 tgtcccagac tacaccaagt aaagatactg atgatcagtc caggaaaaac atgactagca 781 agaaccgggg caagaggaaa gctgatgcca cttcttccca agacagtgaa ttagaacggg 841 tatttctgtg ggacttggat gaaaccatca tcatcttcca ctcacttctt actggatcct 901 atgcccagaa atatggaaag gacccaacag tagtgattgg ctcaggttta acaatggaag 961 aaatgatttt tgaagtggct gatactcatc tatttttcaa tgacttagag gagtgtgacc 1021 aggtacatgt ggaagatgtg gcttctgatg acaatggcca agacttgagc aactacagtt 1081 tctcaacaga tggtttcagt ggctcaggag gtagtggcag ccatggttca tctgtgggtg 1141 ttcagggagg tgtggactgg atgaggaaac tagctttccg ctaccggaaa gtgagagaaa 1201 tctatgataa gcataaaagc aacgtgggtg gtctcctcag tccccagagg aaggaagcac 1261 tgcagagatt aagagcagaa attgaagttt taacagattc ctggttagga actgcattaa 1321 agtccttact tctcatccag tccagaaaga attgtgtgaa tgttctgatc actaccaccc 1381 agctggttcc agccctggcc aaggttctcc tatatggact aggagaaata tttcctattg 1441 agaacatcta tagtgctacc aaaattggta aggagagctg ctttgagaga attgtgtcaa 1501 ggtttggaaa gaaagtcaca tatgtagtga ttggagatgg acgagatgaa gaaattgcag 1561 ccaaacagca actctatttc ttagacatgg aggcattggg ttgccagcta gaaccaactg 1621 ccctgatact cttcatccag ttaagtggaa accttagcaa ctataataaa ctatgcccaa 1681 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1741 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta