Construct: ORF TRCN0000489799
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021514.1_s317c1
- DNA Barcode:
- TACGGGGGAAATTGGTGCTGCGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EYA3 (2140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489799
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NM_001282560.2 | 100% | 100% | |
| 2 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_011541003.2 | 99.6% | 99.6% | 34_39delAGAAAG |
| 3 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NM_001282561.1 | 96.2% | 90.4% | (many diffs) |
| 4 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_011541004.2 | 95.9% | 90.1% | (many diffs) |
| 5 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_011541001.3 | 92% | 92% | 361_498del |
| 6 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_024453995.1 | 92% | 92% | 361_498del |
| 7 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_011540999.2 | 91.7% | 91.7% | 34_39delAGAAAG;367_504del |
| 8 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_024453987.1 | 91.7% | 91.7% | 34_39delAGAAAG;367_504del |
| 9 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_024454014.1 | 90.1% | 90.1% | 0_1ins159 |
| 10 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NM_001990.4 | 88.7% | 83.5% | (many diffs) |
| 11 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_006710449.3 | 88.4% | 83.3% | (many diffs) |
| 12 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XM_024454013.1 | 82.9% | 82.9% | 0_1ins159;202_339del |
| 13 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NM_001282562.1 | 79.6% | 74.7% | (many diffs) |
| 14 | human | 2140 | EYA3 | EYA transcriptional coactiv... | NR_104214.1 | 73.3% | (many diffs) | |
| 15 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XR_001737039.2 | 63.5% | 1_158del;1102_1169del;1391_1392ins443 | |
| 16 | human | 2140 | EYA3 | EYA transcriptional coactiv... | XR_001737038.1 | 25.6% | (many diffs) | |
| 17 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | NM_210071.2 | 86.7% | 85.6% | (many diffs) |
| 18 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | NM_010166.3 | 83.9% | 82.7% | (many diffs) |
| 19 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | XM_006538537.3 | 79.9% | 79.1% | (many diffs) |
| 20 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | XM_006538538.3 | 77.4% | 76.4% | (many diffs) |
| 21 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | NM_211357.2 | 68.5% | 65.8% | (many diffs) |
| 22 | mouse | 14050 | Eya3 | EYA transcriptional coactiv... | XM_006538540.3 | 49.3% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1677
- ORF length:
- 1608
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaagaagag caagatttac cagagcaacc agtgaaaaaa gccaagatgc 121 aggaatcagg agagcaaact ataagtcaag taagcaatcc agatgtcagt gatcagaagc 181 ctgaaacatc aagccttgct tcaaaccttc ccatgtcaga ggaaattatg acatgcaccg 241 attacatccc tcgctcatcc aatgattata cctcacaaat gtattctgca aaaccttatg 301 cacatattct ctcagttcct gtttcggaaa ctgcttaccc tggacagact caataccaga 361 cactacagca gactcaaccc tatgctgtct accctcaggc aacccaaacg tatggactac 421 ctccttttgc ttcaagcaca aatgccagcc tgatatctac ttcttctaca attgccaata 481 ttccagcagc agcagtagcc agcatctcaa accaggatta tcccacctat actattcttg 541 gtcagaatca gtaccaggcc tgctacccca gctccagctt tggagtcaca ggtcagacta 601 acagtgatgc agagagcacc acattagcag caaccacata ccagtcggag aagcctagtg 661 tcatggcgcc tgcacctgca gcacagagac tttcctctgg agacccttct acaagtccat 721 ctttgtccca gactacacca agtaaagata ctgatgatca gtccaggaaa aacatgacta 781 gcaagaaccg gggcaagagg aaagctgatg ccacttcttc ccaagacagt gaattagaac 841 gggtatttct gtgggacttg gatgaaacca tcatcatctt ccactcactt cttactggat 901 cctatgccca gaaatatgga aaggacccaa cagtagtgat tggctcaggt ttaacaatgg 961 aagaaatgat ttttgaagtg gctgatactc atctattttt caatgactta gaggagtgtg 1021 accaggtaca tgtggaagat gtggcttctg atgacaatgg ccaagacttg agcaactaca 1081 gtttctcaac agatggtttc agtggctcag gaggtagtgg cagccatggt tcatctgtgg 1141 gtgttcaggg aggtgtggac tggatgagga aactagcttt ccgctaccgg aaagtgagag 1201 aaatctatga taagcataaa agcaacgtgg gtggtctcct cagtccccag aggaaggaag 1261 cactgcagag attaagagca gaaattgaag ttttaacaga ttcctggtta ggaactgcat 1321 taaagtccTT ACTTCTCATC CAGTCCAGAA AGAATTGTGT GAATGTTCTG ATCACTACCA 1381 CCCAGCTGGT TCCAGCCCTG GCCAAGGTTC TCCTATATGG ACTAGGAGAA ATATTTCCTA 1441 TTGAGAACAT CTATAGTGCT ACCAAAATTG GTAAGGAGAG CTGCTTTGAG AGAATTGTGT 1501 CAAGGTTTGG AAAGAAAGTC ACATATGTAG TGATTGGAGA TGGACGAGAT GAAGAAATTG 1561 CAGCCAAACA GCAACTCTAT TTCTTAGACA TGGAGGCATT GGGTTGCCAG CTAGAACCAA 1621 CTGCCCTGAT ACTCTTCATC CAGTTAAGTG GAAACCTTAG CAACTATAAT AAACTATGAG 1681 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1741 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1801 TTTATATATC TTGTGGAAAG GACGATACGG GGGAAATTGG TGCTGCGTTA CGCGTTAAGT 1861 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt