Transcript: Mouse NM_177682.3

Mus musculus CCZ1 vacuolar protein trafficking and biogenesis associated (Ccz1), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Ccz1 (231874)
Length:
1786
CDS:
42..1484

Additional Resources:

NCBI RefSeq record:
NM_177682.3
NBCI Gene record:
Ccz1 (231874)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_177682.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000177930 CGTATGAAGAGCTCCATTTAA pLKO.1 958 CDS 100% 15.000 21.000 N Ccz1 n/a
2 TRCN0000285849 CGTATGAAGAGCTCCATTTAA pLKO_005 958 CDS 100% 15.000 21.000 N Ccz1 n/a
3 TRCN0000177377 GCCTAAGTGTAGTCAAATATA pLKO.1 682 CDS 100% 15.000 21.000 N Ccz1 n/a
4 TRCN0000297039 GCCTAAGTGTAGTCAAATATA pLKO_005 682 CDS 100% 15.000 21.000 N Ccz1 n/a
5 TRCN0000198254 GCTCAGCAGAACATCTTTAAA pLKO.1 1493 3UTR 100% 15.000 10.500 N Ccz1 n/a
6 TRCN0000277102 ACTGGACCCTTGAACCTTAAT pLKO_005 885 CDS 100% 13.200 9.240 N Ccz1 n/a
7 TRCN0000277100 ATCCGTCCACCCAGATCTAAT pLKO_005 1244 CDS 100% 13.200 9.240 N Ccz1 n/a
8 TRCN0000434255 GAAATTCTTCCATCGGTATTT pLKO_005 542 CDS 100% 13.200 9.240 N CCZ1 n/a
9 TRCN0000277048 CAACCTTTGTGTTTACCATTA pLKO_005 1522 3UTR 100% 10.800 7.560 N Ccz1 n/a
10 TRCN0000198751 CCCTGAGGTTTCTAGATGTTA pLKO.1 1632 3UTR 100% 5.625 3.938 N Ccz1 n/a
11 TRCN0000181233 CAGTCATGTGACCTACTTGAT pLKO.1 579 CDS 100% 4.950 3.465 N Ccz1 n/a
12 TRCN0000176740 CCAGTTTAAGTTCATCTACTT pLKO.1 1157 CDS 100% 4.950 3.465 N Ccz1 n/a
13 TRCN0000198697 CGGTATTTGCAGACACTACAT pLKO.1 555 CDS 100% 4.950 3.465 N Ccz1 n/a
14 TRCN0000177959 GAAAGCCTAAGTGTAGTCAAA pLKO.1 678 CDS 100% 4.950 3.465 N Ccz1 n/a
15 TRCN0000182667 GCAGTGCTACAGCATGTACAA pLKO.1 452 CDS 100% 4.950 3.465 N Ccz1 n/a
16 TRCN0000128235 GCAGTTCAACAACATCTTCTT pLKO.1 1454 CDS 100% 4.950 3.465 N CCZ1 n/a
17 TRCN0000198114 CCATTACAACTTACTGGTCAT pLKO.1 1537 3UTR 100% 4.050 2.835 N Ccz1 n/a
18 TRCN0000160920 GAGAAATTCTTCCATCGGTAT pLKO.1 540 CDS 100% 4.050 2.835 N CCZ1B n/a
19 TRCN0000422714 TGGTTGTTCGGAATCCTATAA pLKO_005 346 CDS 100% 13.200 18.480 N CCZ1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_177682.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05267 pDONR223 100% 88.5% 95.8% None (many diffs) n/a
2 ccsbBroad304_05267 pLX_304 0% 88.5% 95.8% V5 (many diffs) n/a
3 ccsbBroadEn_08329 pDONR223 99.7% 88.4% 95.6% None (many diffs) n/a
Download CSV