Transcript: Mouse NM_175387.3

Mus musculus RNA binding protein, fox-1 homolog (C. elegans) 2 (Rbfox2), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Rbfox2 (93686)
Length:
6980
CDS:
511..1818

Additional Resources:

NCBI RefSeq record:
NM_175387.3
NBCI Gene record:
Rbfox2 (93686)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_175387.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000074544 CGGGTTCGTAACTTTCGAGAA pLKO.1 1173 CDS 100% 4.050 5.670 N RBFOX2 n/a
2 TRCN0000311693 CGGGTTCGTAACTTTCGAGAA pLKO_005 1173 CDS 100% 4.050 5.670 N RBFOX2 n/a
3 TRCN0000102343 CGACTACATGTCTCTAATATT pLKO.1 1051 CDS 100% 15.000 10.500 N Rbfox2 n/a
4 TRCN0000102340 GCCATTAAAGAGTGTGTAGAA pLKO.1 2865 3UTR 100% 4.950 3.465 N Rbfox2 n/a
5 TRCN0000102344 GCTGTGTATGGTCCTGAGTTA pLKO.1 1348 CDS 100% 4.950 3.465 N Rbfox2 n/a
6 TRCN0000074543 CCTCACTATGTTCTTTGAATA pLKO.1 1941 3UTR 100% 13.200 7.920 N RBFOX2 n/a
7 TRCN0000306861 TTGGCGCTGTGGCGAGTTTAT pLKO_005 1753 CDS 100% 13.200 18.480 N RBFOX2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_175387.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02790 pDONR223 100% 74.1% 65% None (many diffs) n/a
2 ccsbBroad304_02790 pLX_304 0% 74.1% 65% V5 (many diffs) n/a
3 ccsbBroadEn_11745 pDONR223 100% 71% 61.8% None (many diffs) n/a
4 ccsbBroad304_11745 pLX_304 0% 71% 61.8% V5 (many diffs) n/a
5 TRCN0000478244 AAGGTTCGACCCGCACGGCTGATA pLX_317 24.7% 71% 61.8% V5 (many diffs) n/a
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