Transcript: Human NM_001145102.1

Homo sapiens SMAD family member 3 (SMAD3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
SMAD3 (4088)
Length:
5997
CDS:
379..1341

Additional Resources:

NCBI RefSeq record:
NM_001145102.1
NBCI Gene record:
SMAD3 (4088)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001145102.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000020009 CTGTGTGAGTTCGCCTTCAAT pLKO.1 385 CDS 100% 5.625 7.875 N SMAD3 n/a
2 TRCN0000330055 GCCTCAGTGACAGCGCTATTT pLKO_005 983 CDS 100% 13.200 9.240 N SMAD3 n/a
3 TRCN0000330128 TGAGCAGAACAGGTAGTATTA pLKO_005 1604 3UTR 100% 13.200 9.240 N SMAD3 n/a
4 TRCN0000330056 GGATTGAGCTGCACCTGAATG pLKO_005 1247 CDS 100% 10.800 7.560 N SMAD3 n/a
5 TRCN0000353587 TCCGCATGAGCTTCGTCAAAG pLKO_005 1178 CDS 100% 10.800 7.560 N SMAD3 n/a
6 TRCN0000020010 CCGCTGTTCCAGTGTGTCTTA pLKO.1 1320 CDS 100% 4.950 3.465 N SMAD3 n/a
7 TRCN0000020012 CATCTCCTACTACGAGCTGAA pLKO.1 765 CDS 100% 4.050 2.835 N SMAD3 n/a
8 TRCN0000020011 CCCAGCACATAATAACTTGGA pLKO.1 702 CDS 100% 2.640 1.848 N SMAD3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001145102.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06549 pDONR223 100% 75.2% 75.2% None 0_1ins315 n/a
2 ccsbBroad304_06549 pLX_304 52.9% 75.2% 75.2% V5 0_1ins315 n/a
3 TRCN0000469603 CAAACTTGGTTGGCATGCAAAGAT pLX_317 28.1% 75.2% 75.2% V5 0_1ins315 n/a
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