Transcript: Mouse NM_013522.3

Mus musculus FSHD region gene 1 (Frg1), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Frg1 (14300)
Length:
1106
CDS:
158..934

Additional Resources:

NCBI RefSeq record:
NM_013522.3
NBCI Gene record:
Frg1 (14300)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_013522.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419141 TTGGGCGTTCGGATGCAATTG pLKO_005 534 CDS 100% 10.800 15.120 N Frg1 n/a
2 TRCN0000438315 GGCTTTACTGGCCTCAAATAG pLKO_005 598 CDS 100% 13.200 10.560 N Frg1 n/a
3 TRCN0000412803 TGTAATGAAGCAGGCGATATA pLKO_005 632 CDS 100% 13.200 9.240 N Frg1 n/a
4 TRCN0000123438 CAGGAACTATTGCCATTGAAA pLKO.1 327 CDS 100% 5.625 3.938 N Frg1 n/a
5 TRCN0000420749 GAACAATGGGAACCAGTGTTT pLKO_005 563 CDS 100% 4.950 3.465 N Frg1 n/a
6 TRCN0000123435 GCACTGGACAATGGACTGTTT pLKO.1 371 CDS 100% 4.950 3.465 N Frg1 n/a
7 TRCN0000123434 CCAACTTGATATTGTGGGAAT pLKO.1 274 CDS 100% 4.050 2.835 N Frg1 n/a
8 TRCN0000123437 CTATATCCATGCACTGGACAA pLKO.1 361 CDS 100% 4.050 2.835 N Frg1 n/a
9 TRCN0000437155 AGTCCTCCAGAGCAGTTTACT pLKO_005 431 CDS 100% 5.625 3.375 N Frg1 n/a
10 TRCN0000432836 TCAGATCCTGTGCGGAAAGAG pLKO_005 696 CDS 100% 4.950 2.970 N Frg1 n/a
11 TRCN0000123436 CCAAATTGAAAGCTGACCGAT pLKO.1 903 CDS 100% 2.640 1.320 Y Frg1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013522.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00591 pDONR223 100% 90.1% 97.2% None (many diffs) n/a
2 ccsbBroad304_00591 pLX_304 0% 90.1% 97.2% V5 (many diffs) n/a
3 TRCN0000471935 TTCCGACCATTCAACCTACGAGGA pLX_317 7.9% 90.1% 97.2% V5 (many diffs) n/a
4 ccsbBroadEn_13507 pDONR223 100% 36.3% 35.6% None (many diffs) n/a
5 ccsbBroad304_13507 pLX_304 0% 36.3% 35.6% V5 (many diffs) n/a
6 TRCN0000475567 TTCGAAGTGTCGGAGGTTAAGAGA pLX_317 72.9% 36.3% 35.6% V5 (many diffs) n/a
Download CSV