Transcript: Mouse NM_001136072.2

Mus musculus Meis homeobox 2 (Meis2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Meis2 (17536)
Length:
4716
CDS:
246..1430

Additional Resources:

NCBI RefSeq record:
NM_001136072.2
NBCI Gene record:
Meis2 (17536)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001136072.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000304270 GACGGGCTCATCGAGACAATT pLKO_005 1930 3UTR 100% 13.200 18.480 N Meis2 n/a
2 TRCN0000075589 CCACGAACTATGTGATAACTT pLKO.1 737 CDS 100% 5.625 7.875 N Meis2 n/a
3 TRCN0000301408 CCACGAACTATGTGATAACTT pLKO_005 737 CDS 100% 5.625 7.875 N Meis2 n/a
4 TRCN0000075588 GCACCATAAGTAGGATTCTAT pLKO.1 2544 3UTR 100% 5.625 7.875 N Meis2 n/a
5 TRCN0000274057 TCTATGGGCACCCGTTGTTTC pLKO_005 484 CDS 100% 10.800 8.640 N MEIS2 n/a
6 TRCN0000310875 TCTATGGGCACCCGTTGTTTC pLKO_005 484 CDS 100% 10.800 8.640 N Meis2 n/a
7 TRCN0000348996 CAAACCCAGAGCTGGATAATT pLKO_005 661 CDS 100% 15.000 10.500 N Meis2 n/a
8 TRCN0000075590 CCCACAATGTTAAATTCTGTA pLKO.1 1688 3UTR 100% 4.950 3.465 N Meis2 n/a
9 TRCN0000301327 CCCACAATGTTAAATTCTGTA pLKO_005 1688 3UTR 100% 4.950 3.465 N Meis2 n/a
10 TRCN0000016044 CCCATGATTGACCAGTCAAAT pLKO.1 1254 CDS 100% 13.200 7.920 N MEIS2 n/a
11 TRCN0000274060 CCCATGATTGACCAGTCAAAT pLKO_005 1254 CDS 100% 13.200 7.920 N MEIS2 n/a
12 TRCN0000075592 CCGGTACATTAGCTGTTTGAA pLKO.1 764 CDS 100% 5.625 3.375 N Meis2 n/a
13 TRCN0000075591 CCACCGGTACATTAGCTGTTT pLKO.1 761 CDS 100% 4.950 2.970 N Meis2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001136072.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00999 pDONR223 100% 91.3% 96.7% None (many diffs) n/a
2 ccsbBroad304_00999 pLX_304 0% 91.3% 96.7% V5 (many diffs) n/a
3 TRCN0000470691 ACACACCCAATCGGACACGTGCAG pLX_317 35% 91.3% 96.7% V5 (many diffs) n/a
4 ccsbBroadEn_00998 pDONR223 100% 77.9% 81.1% None (many diffs) n/a
5 ccsbBroad304_00998 pLX_304 0% 77.9% 81.1% V5 (many diffs) n/a
6 TRCN0000465719 ACTGCGGCTGATCAGATCCCCAGG pLX_317 23% 77.9% 81.1% V5 (many diffs) n/a
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