Transcript: Human XM_011510724.2

PREDICTED: Homo sapiens methyltransferase like 21A (METTL21A), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
METTL21A (151194)
Length:
2488
CDS:
72..857

Additional Resources:

NCBI RefSeq record:
XM_011510724.2
NBCI Gene record:
METTL21A (151194)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011510724.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000263894 ATTCGCTATGAACGGGATAAC pLKO_005 723 CDS 100% 10.800 15.120 N METTL21A n/a
2 TRCN0000263895 CAAACGTTCAAGCCAACTTAC pLKO_005 511 CDS 100% 10.800 15.120 N METTL21A n/a
3 TRCN0000263896 GAGGACTTATAATTGGCTATA pLKO_005 846 CDS 100% 10.800 15.120 N METTL21A n/a
4 TRCN0000263892 CTTGGTGCTGATATCATATAT pLKO_005 621 CDS 100% 15.000 10.500 N METTL21A n/a
5 TRCN0000263893 GCCTTAATTTCAGGTATATAT pLKO_005 1416 3UTR 100% 15.000 10.500 N METTL21A n/a
6 TRCN0000167477 GAAGAAACATTCACAGATCTT pLKO.1 645 CDS 100% 4.950 3.465 N METTL21A n/a
7 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 1690 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011510724.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09678 pDONR223 100% 83.2% 83.1% None (many diffs) n/a
2 TRCN0000468870 AATCTGTCAGAGGCGTTTCTTGGA pLX_317 68.8% 83.2% 83.1% V5 (many diffs) n/a
3 ccsbBroadEn_13273 pDONR223 100% 33.6% 32.9% None (many diffs) n/a
4 ccsbBroad304_13273 pLX_304 0% 33.6% 32.9% V5 (many diffs) n/a
5 TRCN0000467292 CCCTATGAAGGCCTGAAGCGGGCA pLX_317 38.2% 33.6% 32.9% V5 (many diffs) n/a
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