Transcript: Human XM_017000579.1

PREDICTED: Homo sapiens CREB regulated transcription coactivator 2 (CRTC2), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CRTC2 (200186)
Length:
1970
CDS:
73..1536

Additional Resources:

NCBI RefSeq record:
XM_017000579.1
NBCI Gene record:
CRTC2 (200186)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017000579.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218012 CCTAAAGCACTTGTAACTTTG pLKO_005 1749 3UTR 100% 10.800 7.560 N CRTC2 n/a
2 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 27 5UTR 100% 5.625 2.813 Y KLHL30 n/a
3 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 27 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017000579.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09804 pDONR223 100% 69.8% 69.5% None (many diffs) n/a
2 ccsbBroad304_09804 pLX_304 0% 69.8% 69.5% V5 (many diffs) n/a
3 TRCN0000478076 ATCGTATTCAACCATGAACAGTCC pLX_317 15.3% 69.8% 69.5% V5 (many diffs) n/a
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