Transcript: Mouse XM_006515113.3

PREDICTED: Mus musculus phosphoinositide-3-kinase, catalytic, gamma polypeptide (Pik3cg), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pik3cg (30955)
Length:
4241
CDS:
350..3493

Additional Resources:

NCBI RefSeq record:
XM_006515113.3
NBCI Gene record:
Pik3cg (30955)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006515113.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361735 TGCCACAACGATCGCTCAAAT pLKO_005 3001 CDS 100% 13.200 18.480 N Pik3cg n/a
2 TRCN0000368732 TACGCATCATGGAGTCCATTT pLKO_005 2892 CDS 100% 10.800 15.120 N Pik3cg n/a
3 TRCN0000024573 CCACAGTCCTATCCAACGAAA pLKO.1 2808 CDS 100% 4.950 6.930 N Pik3cg n/a
4 TRCN0000024571 CCCAAGTTATTTCACAGCTTA pLKO.1 2631 CDS 100% 4.950 6.930 N Pik3cg n/a
5 TRCN0000024572 GCTGTTAATAGACCACCGTTT pLKO.1 1747 CDS 100% 4.050 5.670 N Pik3cg n/a
6 TRCN0000361703 CAAGATCAGAGGCATTGATAT pLKO_005 1438 CDS 100% 13.200 9.240 N Pik3cg n/a
7 TRCN0000232401 CATAGCCACAGATCCACTTAA pLKO_005 2023 CDS 100% 13.200 9.240 N Pik3cg n/a
8 TRCN0000232402 GACGTCAGTTCCCAAGTTATT pLKO_005 2621 CDS 100% 13.200 9.240 N Pik3cg n/a
9 TRCN0000024570 CCTGCATTACTGGAAGTTGAT pLKO.1 712 CDS 100% 4.950 3.465 N Pik3cg n/a
10 TRCN0000232403 GTACCCTGGTGATCGAGAAAT pLKO_005 2730 CDS 100% 0.000 0.000 N Pik3cg n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006515113.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14762 pDONR223 0% 81.4% 86.5% None (many diffs) n/a
2 ccsbBroad304_14762 pLX_304 0% 81.4% 86.5% V5 (many diffs) n/a
3 TRCN0000480523 TACTATCAGTCTAGTTATCATTTA pLX_317 12.4% 81.4% 86.5% V5 (many diffs) n/a
4 ccsbBroadEn_06727 pDONR223 100% 81.4% 86.4% None (many diffs) n/a
5 ccsbBroad304_06727 pLX_304 0% 81.4% 86.4% V5 (many diffs) n/a
6 TRCN0000481299 CCCACTCTGTAACCGTTGCGGCCC pLX_317 13.1% 81.4% 86.4% V5 (many diffs) n/a
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