Transcript: Mouse XM_017322094.1

PREDICTED: Mus musculus potassium inwardly-rectifying channel, subfamily J, member 14 (Kcnj14), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kcnj14 (211480)
Length:
3164
CDS:
691..1995

Additional Resources:

NCBI RefSeq record:
XM_017322094.1
NBCI Gene record:
Kcnj14 (211480)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017322094.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000069279 CGCCACTTCCATCGAACATAT pLKO.1 1723 CDS 100% 13.200 10.560 N Kcnj14 n/a
2 TRCN0000427941 GGTTGAACATCAGTGAGATAC pLKO_005 2274 3UTR 100% 10.800 7.560 N Kcnj14 n/a
3 TRCN0000069282 CCACAGCCCTAAGTCAAGTTT pLKO.1 1806 CDS 100% 5.625 3.938 N Kcnj14 n/a
4 TRCN0000069278 CCACAGATAGACCACACCTTT pLKO.1 2133 3UTR 100% 4.950 3.465 N Kcnj14 n/a
5 TRCN0000069808 CCTTTATTTCTCTGCTCCATT pLKO.1 2149 3UTR 100% 4.950 3.465 N Gm5522 n/a
6 TRCN0000069280 GAGCCAGGAAACAGTCGAGCT pLKO.1 733 CDS 100% 0.720 0.504 N Kcnj14 n/a
7 TRCN0000044577 GCGCTGGATGTGCCTGCTCTT pLKO.1 942 CDS 100% 0.000 0.000 N KCNJ14 n/a
8 TRCN0000069281 CCAGGATGTAGATGTTGGTTT pLKO.1 1440 CDS 100% 4.950 2.970 N Kcnj14 n/a
9 TRCN0000178741 CACACACATACACACACACAA pLKO.1 206 5UTR 100% 4.950 2.475 Y Cstad n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017322094.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00897 pDONR223 100% 88.1% 94.2% None (many diffs) n/a
2 ccsbBroad304_00897 pLX_304 0% 88.1% 94.2% V5 (many diffs) n/a
3 TRCN0000478066 TTGCCTTCCAGAGATTTTCTGTTT pLX_317 10% 88.1% 94.2% V5 (many diffs) n/a
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