Transcript: Human NM_006353.3

Homo sapiens high mobility group nucleosomal binding domain 4 (HMGN4), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
HMGN4 (10473)
Length:
1943
CDS:
217..489

Additional Resources:

NCBI RefSeq record:
NM_006353.3
NBCI Gene record:
HMGN4 (10473)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006353.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000115688 TCAGCTCGGTTGTCTGCTAAA pLKO.1 289 CDS 100% 10.800 15.120 N HMGN4 n/a
2 TRCN0000289635 TCAGCTCGGTTGTCTGCTAAA pLKO_005 289 CDS 100% 10.800 15.120 N HMGN4 n/a
3 TRCN0000233401 TGGTTGTCTCTGCACTATTTA pLKO_005 1420 3UTR 100% 15.000 10.500 N HMGN4 n/a
4 TRCN0000233400 TGCCAAGTGAAATGTACATTT pLKO_005 480 CDS 100% 13.200 9.240 N HMGN4 n/a
5 TRCN0000115687 CCTCCATATTACTTCTAGAAT pLKO.1 1731 3UTR 100% 5.625 3.938 N HMGN4 n/a
6 TRCN0000234488 GATGAGCCACAGAGGAGATCA pLKO_005 271 CDS 100% 4.950 3.465 N HMGN4 n/a
7 TRCN0000233398 GGATGGGAACAACCCTGCAAA pLKO_005 408 CDS 100% 4.950 3.465 N HMGN4 n/a
8 TRCN0000115691 TCTACACTCCAGTCCCAGAAA pLKO.1 442 CDS 100% 4.950 3.465 N HMGN4 n/a
9 TRCN0000233399 TCTACACTCCAGTCCCAGAAA pLKO_005 442 CDS 100% 4.950 3.465 N HMGN4 n/a
10 TRCN0000115689 CTCTGCAAAGAAGGGAGAGAA pLKO.1 348 CDS 100% 4.950 2.970 N HMGN4 n/a
11 TRCN0000115690 GATAAAGCAAAGGTGAAGGAT pLKO.1 253 CDS 100% 3.000 1.500 Y HMGN4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006353.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07617 pDONR223 100% 99.6% 100% None 198G>A n/a
2 ccsbBroad304_07617 pLX_304 0% 99.6% 100% V5 198G>A n/a
3 TRCN0000478291 GACTAATCTACCGGTGAGCGGTTG pLX_317 100% 99.6% 100% V5 198G>A n/a
4 ccsbBroadEn_00754 pDONR223 100% 86.2% 86.6% None (many diffs) n/a
5 ccsbBroad304_00754 pLX_304 0% 86.2% 86.6% V5 (many diffs) n/a
6 TRCN0000465397 CGTGTAGCACCTTCTGGCTCTCTA pLX_317 100% 86.2% 86.6% V5 (many diffs) n/a
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