Transcript: Human NM_001304717.5

Homo sapiens phosphatase and tensin homolog (PTEN), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
PTEN (5728)
Length:
8514
CDS:
326..2056

Additional Resources:

NCBI RefSeq record:
NM_001304717.5
NBCI Gene record:
PTEN (5728)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001304717.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230371 ATTTCGGGCACCGCATATTAA pLKO_005 4218 3UTR 100% 15.000 21.000 N PTEN n/a
2 TRCN0000230372 TGCTGTGGCACAGGTTATAAA pLKO_005 4805 3UTR 100% 15.000 10.500 N PTEN n/a
3 TRCN0000219043 GGGCTTTAACTGTAGTATTTG pLKO_005 5030 3UTR 100% 13.200 9.240 N PTEN n/a
4 TRCN0000002745 AGGCGCTATGTGTATTATTAT pLKO.1 1358 CDS 100% 15.000 9.000 N PTEN n/a
5 TRCN0000355843 GACGAACTGGTGTAATGATAT pLKO_005 1230 CDS 100% 13.200 7.920 N PTEN n/a
6 TRCN0000002749 CCACAAATGAAGGGATATAAA pLKO.1 2809 3UTR 100% 15.000 7.500 Y PTEN n/a
7 TRCN0000230370 CCACAAATGAAGGGATATAAA pLKO_005 2809 3UTR 100% 15.000 7.500 Y PTEN n/a
8 TRCN0000355842 GACTTAGACTTGACCTATATT pLKO_005 908 CDS 100% 15.000 7.500 Y PTEN n/a
9 TRCN0000230369 TATACCATCTCCAGCTATTTA pLKO_005 2611 3UTR 100% 15.000 7.500 Y PTEN n/a
10 TRCN0000355841 ACAGTAGAGGAGCCGTCAAAT pLKO_005 1892 CDS 100% 13.200 6.600 Y PTEN n/a
11 TRCN0000322487 ACATTATGACACCGCCAAATT pLKO_005 1066 CDS 100% 13.200 6.600 Y Pten n/a
12 TRCN0000355946 ACATTATGACACCGCCAAATT pLKO_005 1066 CDS 100% 13.200 6.600 Y PTEN n/a
13 TRCN0000028991 CGACTTAGACTTGACCTATAT pLKO.1 907 CDS 100% 13.200 6.600 Y Pten n/a
14 TRCN0000322421 CGACTTAGACTTGACCTATAT pLKO_005 907 CDS 100% 13.200 6.600 Y Pten n/a
15 TRCN0000028990 GCCAGCTAAAGGTGAAGATAT pLKO.1 1497 CDS 100% 13.200 6.600 Y Pten n/a
16 TRCN0000355840 GGCACAAGAGGCCCTAGATTT pLKO_005 1285 CDS 100% 13.200 6.600 Y PTEN n/a
17 TRCN0000322423 CAATATTGATGATGTAGTAAG pLKO_005 988 CDS 100% 10.800 5.400 Y Pten n/a
18 TRCN0000322424 TTGTGGCAACAGATAAGTTTG pLKO_005 2474 3UTR 100% 10.800 5.400 Y Pten n/a
19 TRCN0000002746 CCACAGCTAGAACTTATCAAA pLKO.1 1130 CDS 100% 5.625 2.813 Y PTEN n/a
20 TRCN0000002747 CTAGAACTTATCAAACCCTTT pLKO.1 1136 CDS 100% 4.050 2.025 Y PTEN n/a
21 TRCN0000002748 CGTGCAGATAATGACAAGGAA pLKO.1 1766 CDS 100% 3.000 1.500 Y PTEN n/a
22 TRCN0000028992 GCTAGAACTTATCAAACCCTT pLKO.1 1135 CDS 100% 2.640 1.320 Y Pten n/a
23 TRCN0000322486 GCTAGAACTTATCAAACCCTT pLKO_005 1135 CDS 100% 2.640 1.320 Y Pten n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001304717.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487886 CATTTCCCTCTCTTACTCCAATTC pLX_317 25.6% 69.9% 69.9% V5 1_519del n/a
2 TRCN0000489383 AGAAAGGTTCTACATGCGCCGCGG pLX_317 32.6% 69.9% 69.9% V5 (not translated due to prior stop codon) 1_519del n/a
3 ccsbBroadEn_10499 pDONR223 100% 63% 62.3% None (many diffs) n/a
4 ccsbBroad304_10499 pLX_304 0% 63% 62.3% V5 (many diffs) n/a
5 TRCN0000479391 GCCAATCGATACGCGGTAGTTGCT pLX_317 32.1% 63% 62.3% V5 (many diffs) n/a
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