Transcript: Human NM_012405.4

Homo sapiens isoprenylcysteine carboxyl methyltransferase (ICMT), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
ICMT (23463)
Length:
4795
CDS:
54..908

Additional Resources:

NCBI RefSeq record:
NM_012405.4
NBCI Gene record:
ICMT (23463)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_012405.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000310659 ACGGGCCTGCCTTTCATAAAG pLKO_005 867 CDS 100% 13.200 18.480 N ICMT n/a
2 TRCN0000303341 CATGGGTGAAAGACGAGTAAG pLKO_005 1119 3UTR 100% 10.800 8.640 N ICMT n/a
3 TRCN0000303342 ATAGCCATCCGAGCTTGTTTC pLKO_005 249 CDS 100% 10.800 7.560 N ICMT n/a
4 TRCN0000035272 GATCGAACAGAAGAAGAAGAA pLKO.1 789 CDS 100% 4.950 3.465 N ICMT n/a
5 TRCN0000291665 GATCGAACAGAAGAAGAAGAA pLKO_005 789 CDS 100% 4.950 3.465 N ICMT n/a
6 TRCN0000035269 GCTGCTCTTTCTTCTTGGTTA pLKO.1 456 CDS 100% 4.950 3.465 N ICMT n/a
7 TRCN0000291666 GCTGCTCTTTCTTCTTGGTTA pLKO_005 456 CDS 100% 4.950 3.465 N ICMT n/a
8 TRCN0000035270 CTTCGGAGAATGTCTGAGGAA pLKO.1 557 CDS 100% 2.640 1.848 N ICMT n/a
9 TRCN0000035273 CCTGGAGTATAAGAAGAGGGT pLKO.1 842 CDS 100% 0.660 0.462 N ICMT n/a
10 TRCN0000295336 GATGGTGGTCTTCGGAGAATG pLKO_005 548 CDS 100% 10.800 6.480 N Icmt n/a
11 TRCN0000035271 GTTCCACTATTCTGAATACTT pLKO.1 359 CDS 100% 5.625 3.375 N ICMT n/a
12 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 3770 3UTR 100% 4.950 2.475 Y LOC387873 n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3807 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3807 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_012405.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15012 pDONR223 97.1% 100% 100% None n/a
2 ccsbBroad304_15012 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000471390 TTCTCCATATGTATTAAAACCCTC pLX_317 55.8% 100% 100% V5 n/a
4 ccsbBroadEn_02772 pDONR223 100% 100% 100% None n/a
5 ccsbBroad304_02772 pLX_304 0% 100% 100% V5 n/a
6 TRCN0000475593 TGCTAAATATTCTGCCCGACCCTC pLX_317 32.5% 100% 100% V5 n/a
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