Transcript: Human XM_005250655.2

PREDICTED: Homo sapiens transmembrane protein 209 (TMEM209), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TMEM209 (84928)
Length:
3537
CDS:
417..2099

Additional Resources:

NCBI RefSeq record:
XM_005250655.2
NBCI Gene record:
TMEM209 (84928)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005250655.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000115888 CCTACGAACTTTGGATACTTT pLKO.1 1127 CDS 100% 5.625 7.875 N TMEM209 n/a
2 TRCN0000292136 CCTACGAACTTTGGATACTTT pLKO_005 1127 CDS 100% 5.625 7.875 N TMEM209 n/a
3 TRCN0000115889 CCGACTTTGAACACAATCGTT pLKO.1 1587 CDS 100% 3.000 4.200 N TMEM209 n/a
4 TRCN0000307961 CCGACTTTGAACACAATCGTT pLKO_005 1587 CDS 100% 3.000 4.200 N TMEM209 n/a
5 TRCN0000115887 GCTTCCATCATCTTATGTAAA pLKO.1 3300 3UTR 100% 13.200 10.560 N TMEM209 n/a
6 TRCN0000292138 GCTTCCATCATCTTATGTAAA pLKO_005 3300 3UTR 100% 13.200 10.560 N TMEM209 n/a
7 TRCN0000115890 GCATCTCTCTTCAGCCTTAAT pLKO.1 624 CDS 100% 13.200 9.240 N TMEM209 n/a
8 TRCN0000307960 GCATCTCTCTTCAGCCTTAAT pLKO_005 624 CDS 100% 13.200 9.240 N TMEM209 n/a
9 TRCN0000115891 CCAGATGTTACAAATGAGAAT pLKO.1 1869 CDS 100% 4.950 3.465 N TMEM209 n/a
10 TRCN0000292137 CCAGATGTTACAAATGAGAAT pLKO_005 1869 CDS 100% 4.950 3.465 N TMEM209 n/a
11 TRCN0000183287 GCTGGAATGATATATACTGAA pLKO.1 531 CDS 100% 4.950 3.465 N Tmem209 n/a
12 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 2659 3UTR 100% 4.950 2.475 Y n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005250655.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12883 pDONR223 100% 92.2% 91.9% None (many diffs) n/a
2 ccsbBroad304_12883 pLX_304 0% 92.2% 91.9% V5 (many diffs) n/a
3 TRCN0000479999 TGCCCGCCCAAAGGTGGAGGATCC pLX_317 27.9% 92.2% 91.9% V5 (many diffs) n/a
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