Construct: ORF TRCN0000479999
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015334.2_s317c1
- Derived from:
- ccsbBroadEn_12883
- DNA Barcode:
- TGCCCGCCCAAAGGTGGAGGATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM209 (84928)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479999
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84928 | TMEM209 | transmembrane protein 209 | NM_001301163.2 | 99.8% | 99.6% | 1280C>G;1388A>G |
| 2 | human | 84928 | TMEM209 | transmembrane protein 209 | NM_032842.4 | 92.3% | 92.1% | 1119_1244del;1406C>G;1514A>G |
| 3 | human | 84928 | TMEM209 | transmembrane protein 209 | NM_001363478.2 | 92.2% | 91.9% | (many diffs) |
| 4 | human | 84928 | TMEM209 | transmembrane protein 209 | XM_005250655.2 | 92.2% | 91.9% | (many diffs) |
| 5 | human | 84928 | TMEM209 | transmembrane protein 209 | NR_156699.2 | 44.6% | (many diffs) | |
| 6 | mouse | 72649 | Tmem209 | transmembrane protein 209 | NM_178625.4 | 82.3% | 87.8% | (many diffs) |
| 7 | mouse | 72649 | Tmem209 | transmembrane protein 209 | NM_001311093.1 | 82.1% | 87.7% | (many diffs) |
| 8 | mouse | 72649 | Tmem209 | transmembrane protein 209 | XM_006505170.3 | 82.1% | 87.7% | (many diffs) |
| 9 | mouse | 72649 | Tmem209 | transmembrane protein 209 | XM_006505171.2 | 81% | 86.3% | (many diffs) |
| 10 | mouse | 72649 | Tmem209 | transmembrane protein 209 | XM_006505172.2 | 75.4% | 80.3% | (many diffs) |
| 11 | mouse | 72649 | Tmem209 | transmembrane protein 209 | NM_001347087.1 | 57.3% | 62% | (many diffs) |
| 12 | mouse | 72649 | Tmem209 | transmembrane protein 209 | XM_017321766.1 | 57.3% | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1623
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gcagggggag gcacacccta gtgcttccct tattgacaga accatcaaga 121 tgagaaaaga aacagaggct aggaaagtgg tcttagcctg gggactccta aatgtatcta 181 tggctggaat gatatatact gaaatgactg gaaaattgat tagttcatac tacaatgtga 241 catactggcc cctctggtat attgagcttg cccttgcatc tctcttcagc cttaatgcct 301 tatttgattt ttggagatat ttcaaatata ctgtggcacc aacaagtctg gttgttagtc 361 ctggacagca aacactttta gggttgaaaa cagctgttgt acagactacg cctccacatg 421 atctggcagc aacccaaatc cctcccgctc caccttcccc ttcaattcag ggtcagagtg 481 tgttgagtta tagcccttct cgttcgccca gtaccagtcc caagttcacc accagctgta 541 tgactggtta cagccctcag ctgcaaggtc tgtcctcagg tggcagtggt tcttatagcc 601 ctggagtgac ctactcgccc gtcagtggtt ataataagtt ggcgagcttt agcccctctc 661 ctccttctcc gtaccctacc actgttggac cagtggagag cagtggattg agatctcgct 721 accgttcttc acctaccgtc tacaactcac ctactgacaa agaagactac atgaccgacc 781 tacgaacttt ggatactttt ctcagaagtg aagaggagaa acagcatagg gttaagctgg 841 ggagcccaga ttctacctct ccttccagca gtcctacttt ctggaactat agtcgttcta 901 tgggggatta tgcacaaact ttaaagaagt ttcagtatca gcttgcctgt aggtctcagg 961 ccccatgtgc taacaaagat gaagccgatc tcagctctaa acaagccgca gaagaggtct 1021 gggcaagagt ggctatgaat agacaacttc ttgatcatat ggattcatgg acagctaaat 1081 ttagaaattg gatcaatgag acaatattag tgccacttgt tcaagagatt gagtctgtca 1141 gcacacagat gagacgaatg ggttgtccag agctacagat aggagaacta tcTCAGGGAG 1201 GTTGTATGAG CTCATTTCGA TGGAACAGAG GTGGCGACTT CAAAGGACGA AAGTGGGATA 1261 CAGACCTGCC CACCGATTCT GCTATCATCA TGCATGTATT TTGCACCTAC CTTGATTCCA 1321 GATTACCTCC ACATCCGAAG TATCGCGACG GAAAAACTTT TACTTCTCAG CACTTTGTTC 1381 AGACACCAAA TAAACCAGAT GTTACAAATG AGAATGTTTT TTGCATTTAT CAGAGTGCTA 1441 TCAACCCTCC CCGTTATGAG CTCATCTACC AGCGTCATGT ATACAACCTG CCAAAGGGCA 1501 GAAATAATAT GTTTCATACA TTGTTGATGT TTCTCTACAT CATAAAGACC AAAGAGTCAG 1561 GAATGCTTGG GAGAGTTAAT CTTGGTCTAT CTGGTGTGAA TATATTGTGG ATCTTTGGCG 1621 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGATG CCCGCCCAAA GGTGGAGGAT CCACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt