Transcript: Human XM_005272169.2

PREDICTED: Homo sapiens adenylate kinase 8 (AK8), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AK8 (158067)
Length:
1649
CDS:
60..1535

Additional Resources:

NCBI RefSeq record:
XM_005272169.2
NBCI Gene record:
AK8 (158067)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149503 GTATCATAGGAACATCGTCA pXPR_003 GGG 715 48% 8 1.2644 AK8 AK8 77883
2 BRDN0001146232 TGAACGGGGCATTAGTACGA pXPR_003 TGG 821 56% 9 1.0717 AK8 AK8 77884
3 BRDN0001149279 TTCGGGTGGCCAGTCAAAGG pXPR_003 TGG 610 41% 8 0.6373 AK8 AK8 77882
4 BRDN0001148581 CTGGATGAGCTCGCCAAACG pXPR_003 TGG 973 66% 10 -0.0031 AK8 AK8 77885
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005272169.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358967 CAAAGCAACCATCGTACTAAT pLKO_005 867 CDS 100% 13.200 18.480 N AK8 n/a
2 TRCN0000358966 CATCGAGAGTGGGATCATTAA pLKO_005 1490 CDS 100% 13.200 18.480 N AK8 n/a
3 TRCN0000078679 CCAGCACTTGCATAGAGACAA pLKO.1 236 CDS 100% 4.950 6.930 N AK8 n/a
4 TRCN0000078680 CCCTCAAACTGGAGAGATTTA pLKO.1 638 CDS 100% 13.200 9.240 N AK8 n/a
5 TRCN0000359047 TCACACCCAGACACGTCATTG pLKO_005 562 CDS 100% 10.800 7.560 N AK8 n/a
6 TRCN0000078682 CTGACTCTGAGAAGAATTGAT pLKO.1 1260 CDS 100% 5.625 3.938 N AK8 n/a
7 TRCN0000078678 GCTGACTTGGAGCAGTTGTAT pLKO.1 1416 CDS 100% 5.625 3.938 N AK8 n/a
8 TRCN0000078681 GCACTTGCATAGAGACAACGA pLKO.1 239 CDS 100% 2.640 1.848 N AK8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005272169.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05092 pDONR223 100% 97.5% 97.5% None 82_117del n/a
2 ccsbBroad304_05092 pLX_304 0% 97.5% 97.5% V5 82_117del n/a
3 ccsbBroadEn_15271 pDONR223 0% 97.5% 97.5% None 82_117del n/a
4 ccsbBroad304_15271 pLX_304 0% 97.5% 97.5% V5 82_117del n/a
5 TRCN0000480307 CCATCGTTTCGTGATACCTGTAGT pLX_317 30.2% 97.5% 97.5% V5 (not translated due to frame shift) 82_117del n/a
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