Transcript: Human XM_005274337.3

PREDICTED: Homo sapiens phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant X25, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PICALM (8301)
Length:
3520
CDS:
260..2053

Additional Resources:

NCBI RefSeq record:
XM_005274337.3
NBCI Gene record:
PICALM (8301)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005274337.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000119074 CTACATTTATTAGGCGGTATA pLKO.1 621 CDS 100% 10.800 15.120 N PICALM n/a
2 TRCN0000230157 GGCAATCTTGGCATCGGAAAT pLKO_005 1904 CDS 100% 10.800 15.120 N PICALM n/a
3 TRCN0000119075 CCTAGCAAGTTAGTATCTGAT pLKO.1 1853 CDS 100% 4.950 6.930 N PICALM n/a
4 TRCN0000119076 CGTATCCAAGACAGTATGCAA pLKO.1 322 CDS 100% 3.000 4.200 N PICALM n/a
5 TRCN0000230156 GCACCAGCCATTGACATATTT pLKO_005 1370 CDS 100% 15.000 10.500 N PICALM n/a
6 TRCN0000313326 GCACCAGCCATTGACATATTT pLKO_005 1370 CDS 100% 15.000 10.500 N Picalm n/a
7 TRCN0000230155 GAATTGACAGAGGTGATATAC pLKO_005 1032 CDS 100% 13.200 9.240 N PICALM n/a
8 TRCN0000119072 GCCTTAATGTTGACTTTGAAT pLKO.1 1710 CDS 100% 5.625 3.938 N PICALM n/a
9 TRCN0000113186 GCAGCATACAATGAAGGAATT pLKO.1 884 CDS 100% 0.000 0.000 N Picalm n/a
10 TRCN0000218840 TGCCATGTTATGATCATATAC pLKO_005 3110 3UTR 100% 13.200 7.920 N PICALM n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005274337.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01884 pDONR223 100% 82.7% 82.4% None (many diffs) n/a
2 ccsbBroad304_01884 pLX_304 0% 82.7% 82.4% V5 (many diffs) n/a
3 TRCN0000480673 ATGGTAAACCCTCTATTCGATGTC pLX_317 18.6% 82.7% 82.4% V5 (many diffs) n/a
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