Construct: ORF TRCN0000480673
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006272.1_s317c1
- Derived from:
- ccsbBroadEn_01884
- DNA Barcode:
- ATGGTAAACCCTCTATTCGATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PICALM (8301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480673
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 8301 | PICALM | phosphatidylinositol bindin... | NM_001008660.3 | 100% | 100% | |
2 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274336.3 | 99.1% | 99.1% | 1365_1366insGGCTTTGATGAACTA |
3 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_017018383.2 | 98.6% | 98.6% | 1629_1630ins24 |
4 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_017018384.2 | 97.8% | 97.8% | 1365_1366insGGCTTTGATGAACTA;1614_1615ins24 |
5 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274330.3 | 95.7% | 95.2% | (many diffs) |
6 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_017018386.2 | 95.4% | 95.4% | 1629_1630ins84 |
7 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274332.3 | 94.9% | 94.4% | (many diffs) |
8 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_017018387.2 | 94.5% | 94.5% | 1365_1366insGGCTTTGATGAACTA;1614_1615ins84 |
9 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274333.3 | 94.4% | 94% | (many diffs) |
10 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_017018388.2 | 94% | 94% | 1257_1258ins108 |
11 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274335.3 | 93.7% | 93.2% | (many diffs) |
12 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_024448700.1 | 93.4% | 93.4% | 1258_1386del |
13 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_017018381.2 | 92.6% | 92.6% | 1258_1386del;1494_1495insGGCTTTGATGAACTA |
14 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274326.3 | 92.4% | 92.4% | 1258_1407del |
15 | human | 8301 | PICALM | phosphatidylinositol bindin... | NM_001206946.2 | 92.1% | 92.1% | 1258_1386del;1758_1759ins24 |
16 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274328.3 | 91.6% | 91.6% | 1258_1407del;1515_1516insGGCTTTGATGAACTA |
17 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_017018382.2 | 91.3% | 90.8% | (many diffs) |
18 | human | 8301 | PICALM | phosphatidylinositol bindin... | NM_007166.4 | 91.2% | 91.2% | 1258_1407del;1779_1780ins24 |
19 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274329.4 | 90.4% | 90.4% | 1258_1407del;1515_1516insGGCTTTGATGAACTA;1764_1765ins24 |
20 | human | 8301 | PICALM | phosphatidylinositol bindin... | NM_001206947.2 | 90.3% | 90.3% | 0_1ins153;1476_1477ins24 |
21 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_011545293.2 | 90% | 89.6% | (many diffs) |
22 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_006718699.3 | 89.6% | 89.2% | (many diffs) |
23 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274340.3 | 89.5% | 89.1% | (many diffs) |
24 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_006718700.3 | 88.9% | 88.5% | (many diffs) |
25 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274322.3 | 88.7% | 88.3% | (many diffs) |
26 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_006718701.4 | 88.5% | 88% | (many diffs) |
27 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274331.3 | 88.1% | 88.1% | 1258_1407del;1779_1780ins84 |
28 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274323.3 | 88% | 87.6% | (many diffs) |
29 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274324.3 | 87.6% | 87.1% | (many diffs) |
30 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274334.3 | 87.4% | 87.4% | 1258_1407del;1515_1516insGGCTTTGATGAACTA;1764_1765ins84 |
31 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274325.3 | 86.8% | 86.4% | (many diffs) |
32 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274327.3 | 84.6% | 84.2% | (many diffs) |
33 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_005274337.3 | 82.7% | 82.4% | (many diffs) |
34 | human | 8301 | PICALM | phosphatidylinositol bindin... | XM_017018385.2 | 82% | 81.6% | (many diffs) |
35 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | NM_001252522.1 | 94.4% | 98.1% | (many diffs) |
36 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | NM_001252523.1 | 93.6% | 97.3% | (many diffs) |
37 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | NM_001252524.1 | 92.2% | 96% | (many diffs) |
38 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507602.3 | 90.3% | 93.5% | (many diffs) |
39 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507603.3 | 89.5% | 92.7% | (many diffs) |
40 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507604.3 | 89% | 92.2% | (many diffs) |
41 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507605.3 | 88.3% | 91.5% | (many diffs) |
42 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | NM_146194.4 | 87.2% | 90.7% | (many diffs) |
43 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | NM_001252520.1 | 86.5% | 90% | (many diffs) |
44 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | NM_001252521.1 | 86% | 89.5% | (many diffs) |
45 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507601.3 | 85.3% | 88.7% | (many diffs) |
46 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507596.3 | 84.6% | 87.6% | (many diffs) |
47 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507594.3 | 83.7% | 86.7% | (many diffs) |
48 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507599.3 | 83.4% | 86.4% | (many diffs) |
49 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507595.3 | 83% | 86% | (many diffs) |
50 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507600.3 | 82.7% | 85.7% | (many diffs) |
51 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507597.3 | 82.6% | 85.5% | (many diffs) |
52 | mouse | 233489 | Picalm | phosphatidylinositol bindin... | XM_006507598.3 | 81.8% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1899
- ORF length:
- 1830
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccggccag agcctgacgg accgaatcac tgccgcccag cacagtgtca 121 ccggctctgc cgtatccaag acagtatgca aggccacgac ccacgagatc atggggccca 181 agaaaaagca cctggactac ttaattcagt gcacaaatga gatgaatgtg aacatcccac 241 agttggcaga cagtttattt gaaagaacta ctaatagtag ttgggtggtg gtcttcaaat 301 ctctcattac aactcatcat ttgatggtgt atggaaatga gcgttttatt cagtatttgg 361 cttcaagaaa cacgttgttt aacttaagca attttttgga taaaagtgga ttgcaaggat 421 atgacatgtc tacatttatt aggcggtata gtagatattt aaatgagaaa gcagtttcat 481 acagacaagt tgcatttgat ttcacaaaag tgaagagagg ggctgatgga gttatgagaa 541 caatgaacac agaaaaactc ctaaaaactg taccaattat tcagaatcag atggatgcac 601 ttcttgattt taatgttaat agcaatgaac ttacaaatgg ggtaataaat gctgccttca 661 tgctcctgtt caaagatgcc attagactgt ttgcagcata caatgaagga attattaatt 721 tgttggaaaa atattttgat atgaaaaaga accaatgcaa agaaggtctt gacatctata 781 agaagttcct aactaggatg acaagaatct cagagttcct caaagttgca gagcaagttg 841 gaattgacag aggtgatata ccagaccttt cacaggcccc tagcagtctt cttgatgctt 901 tggaacaaca tttagcttcc ttggaaggaa agaaaatcaa agattctaca gctgcaagca 961 gggcaactac actttccaat gcagtgtctt ccctggcaag cactggtcta tctctgacca 1021 aagtggatga aagggaaaag caggcagcat tagaggaaga acaggcacgt ttgaaagctt 1081 taaaggaaca gcgcctaaaa gaacttgcaa agaaacctca tacctcttta acaactgcag 1141 cctctcctgt atccacctca gcaggaggga taatgactgc accagccatt gacatatttt 1201 ctacccctag ttcttctaac agcacatcaa agctgcccaa tgatctgctt gatttgcagc 1261 agccaacttt tcacccatct gtacatccta tgtcaactgc ttctcaggta gcaagtacat 1321 ggggaggatt cactccttct ccagttgcac agccacaccc ttcagctggc cttaatgttg 1381 actttgaatc tgtgtttgga aataaatcta caaatgttat tgtagattct gggggctttg 1441 atgaactagg tggacttctc aaaccaacag tggcctctca gaaccagaac cttcctgttg 1501 ccaaactccc acctagcaag ttagtatctg atgacttgga ttcatcttta gccaaccttg 1561 tgggCAATCT TGGCATCGGA AATGGAACCA CTAAGAATGA TGTAAATTGG AGTCAACCAG 1621 GTGAAAAGAA GTTAACTGGG GGATCTAACT GGCAACCAAA GGTTGCACCA ACAACCGCTT 1681 GGAATGCTGC AACAATGAAT GGCATGCATT TTCCACAATA CGCACCCCCT GTAATGGCCT 1741 ATCCTGCTAC TACACCAACA GGCATGATAG GATATGGAAT TCCTCCACAA ATGGGAAGTG 1801 TTCCTGTAAT GACGCAACCA ACCTTAATAT ACAGCCAGCC TGTCATGAGA CCTCCAAACC 1861 CCTTTGGCCC TGTATCAGGA GCACAGATAC AGTTTATGTT GCCAACTTTC TTGTACAAAG 1921 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1981 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2041 ACGAATGGTA AACCCTCTAT TCGATGTCAC GCGTTAAGTC gacaatcaac ctctggatta 2101 caaaatttgt gaaagatt