Transcript: Mouse XM_006505695.3

PREDICTED: Mus musculus microphthalmia-associated transcription factor (Mitf), transcript variant X9, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mitf (17342)
Length:
4642
CDS:
216..1457

Additional Resources:

NCBI RefSeq record:
XM_006505695.3
NBCI Gene record:
Mitf (17342)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006505695.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305603 TAACATAAACGACCGCATTAA pLKO_005 851 CDS 100% 13.200 18.480 N Mitf n/a
2 TRCN0000095288 GCAAATACGTTACCCGTCTCT pLKO.1 648 CDS 100% 2.640 3.696 N Mitf n/a
3 TRCN0000095287 CCTATGGCTATGCTCACTCTT pLKO.1 435 CDS 100% 4.950 3.960 N Mitf n/a
4 TRCN0000095284 GCCAGACTTGTATATTCTATT pLKO.1 1617 3UTR 100% 13.200 9.240 N Mitf n/a
5 TRCN0000323677 GCCAGACTTGTATATTCTATT pLKO_005 1617 3UTR 100% 13.200 9.240 N Mitf n/a
6 TRCN0000362601 TATGGCTATGCTCACTCTTAA pLKO_005 437 CDS 100% 13.200 9.240 N Mitf n/a
7 TRCN0000362536 TCAGCCTGGAATCAAGTTATA pLKO_005 586 CDS 100% 13.200 9.240 N Mitf n/a
8 TRCN0000095286 CGGTGGAACAAGGGAACCATT pLKO.1 915 CDS 100% 4.950 3.465 N Mitf n/a
9 TRCN0000095285 GCAGTACCTTTCTACCACTTT pLKO.1 314 CDS 100% 4.950 3.465 N Mitf n/a
10 TRCN0000323608 GCAGTACCTTTCTACCACTTT pLKO_005 314 CDS 100% 4.950 3.465 N Mitf n/a
11 TRCN0000087583 AGGAGGAAGAGGAAGAGGAAA pLKO.1 1907 3UTR 100% 4.950 2.475 Y Adam32 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006505695.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01017 pDONR223 100% 88.1% 93.9% None (many diffs) n/a
2 ccsbBroad304_01017 pLX_304 0% 88.1% 93.9% V5 (many diffs) n/a
3 TRCN0000469674 TTCGCAATTCTTGTCATTTGACGT pLX_317 40.3% 88.1% 93.9% V5 (many diffs) n/a
4 ccsbBroadEn_06583 pDONR223 100% 20.5% 20.3% None (many diffs) n/a
5 ccsbBroad304_06583 pLX_304 0% 20.5% 20.3% V5 (many diffs) n/a
6 TRCN0000475375 ACCGGCGCAAAGTGGTACCTTATT pLX_317 100% 20.5% 20.3% V5 (many diffs) n/a
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