Construct: ORF ccsbBroad304_01017

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010217.1_s304c1, BRDN0000403108
Derived from:
ccsbBroadEn_01017
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MITF (4286)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01017
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4286 MITF melanocyte inducing transcr... NM_198158.3 100% 100%
2 human 4286 MITF melanocyte inducing transcr... NM_000248.3 98.5% 98.5% 555_572del
3 human 4286 MITF melanocyte inducing transcr... NM_198178.3 86.4% 86.4% 91_92ins168
4 human 4286 MITF melanocyte inducing transcr... NM_001184967.2 85.9% 85.4% (many diffs)
5 human 4286 MITF melanocyte inducing transcr... NM_001354608.2 85.9% 85.4% (many diffs)
6 human 4286 MITF melanocyte inducing transcr... NM_001354607.2 80% 79.5% (many diffs)
7 human 4286 MITF melanocyte inducing transcr... NM_198177.3 79.8% 79.3% (many diffs)
8 human 4286 MITF melanocyte inducing transcr... NM_001354606.2 77.6% 77% (many diffs)
9 human 4286 MITF melanocyte inducing transcr... NM_006722.2 77.6% 77% (many diffs)
10 human 4286 MITF melanocyte inducing transcr... NM_198159.3 77.4% 76.9% (many diffs)
11 human 4286 MITF melanocyte inducing transcr... NM_001354605.2 76.7% 76.2% (many diffs)
12 human 4286 MITF melanocyte inducing transcr... NM_001354604.2 76.5% 76% (many diffs)
13 human 4286 MITF melanocyte inducing transcr... NM_001184968.2 22% 21.5% (many diffs)
14 mouse 17342 Mitf microphthalmia-associated t... XM_006505695.3 88.1% 93.9% (many diffs)
15 mouse 17342 Mitf microphthalmia-associated t... NM_008601.3 86.9% 92.6% (many diffs)
16 mouse 17342 Mitf microphthalmia-associated t... XM_006505696.1 85% 90.2% (many diffs)
17 mouse 17342 Mitf microphthalmia-associated t... XM_006505692.3 74.9% 79.3% (many diffs)
18 mouse 17342 Mitf microphthalmia-associated t... XM_006505693.1 74.9% 79.3% (many diffs)
19 mouse 17342 Mitf microphthalmia-associated t... XM_006505694.1 74.9% 79.3% (many diffs)
20 mouse 17342 Mitf microphthalmia-associated t... XM_011241244.2 73.4% 79.2% (many diffs)
21 mouse 17342 Mitf microphthalmia-associated t... XM_017321428.1 71.5% 75.6% (many diffs)
22 mouse 17342 Mitf microphthalmia-associated t... XM_006505691.3 71.3% 75.5% (many diffs)
23 mouse 17342 Mitf microphthalmia-associated t... XM_006505689.3 69.8% 73.9% (many diffs)
24 mouse 17342 Mitf microphthalmia-associated t... NM_001178049.1 69.7% 73.7% (many diffs)
25 mouse 17342 Mitf microphthalmia-associated t... XM_006505685.3 68.3% 72.3% (many diffs)
26 mouse 17342 Mitf microphthalmia-associated t... XM_006505684.2 67.7% 71.6% (many diffs)
27 mouse 17342 Mitf microphthalmia-associated t... NM_001113198.1 67.6% 71.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1305
ORF length:
1239
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct ggaaatgcta gaatataatc actatcaggt gcagacccac ctcgaaaacc
121ccaccaagta ccacatacag caagcccaac ggcagcaggt aaagcagtac ctttctacca
181ctttagcaaa taaacatgcc aaccaagtcc tgagcttgcc atgtccaaac cagcctggcg
241atcatgtcat gccaccggtg ccggggagca gcgcacccaa cagccccatg gctatgctta
301cgcttaactc caactgtgaa aaagagggat tttataagtt tgaagagcaa aacagggcag
361agagcgagtg cccaggcatg aacacacatt cacgagcgtc ctgtatgcag atggatgatg
421taatcgatga catcattagc ctagaatcaa gttataatga ggaaatcttg ggcttgatgg
481atcctgcttt gcaaatggca aatacgttgc ctgtctcggg aaacttgatt gatctttatg
541gaaaccaagg tctgccccca ccaggcctca ccatcagcaa ctcctgtcca gccaaccttc
601ccaacataaa aagggagctc acagagtctg aagcaagagc actggccaaa gagaggcaga
661aaaaggacaa tcacaacctg attgaacgaa gaagaagatt taacataaat gaccgcatta
721aagaactagg tactttgatt cccaagtcaa atgatccaga catgcgctgg aacaagggaa
781ccatcttaaa agcatccgtg gactatatcc gaaagttgca acgagaacag caacgcgcaa
841aagaacttga aaaccgacag aagaaactgg agcacgccaa ccggcatttg ttgctcagaa
901tacaggaact tgaaatgcag gctcgagctc atggactttc ccttattcca tccacgggtc
961tctgctctcc agatttggtg aatcggatca tcaagcaaga acccgttctt gagaactgca
1021gccaagacct ccttcagcat catgcagacc taacctgtac aacaactctc gatctcacgg
1081atggcaccat caccttcaac aacaacctcg gaactgggac tgaggccaac caagcctata
1141gtgtccccac aaaaatggga tccaaactgg aagacatcct gatggacgac accctttctc
1201ccgtcggtgt cactgatcca ctcctttcct cagtgtcccc cggagcttcc aaaacaagca
1261gccggaggag cagtatgagc atggaagaga cggagcacac ttgttaccca actttcttgt
1321acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1381gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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