Construct: ORF TRCN0000475375
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008288.1_s317c1
- Derived from:
- ccsbBroadEn_06583
- DNA Barcode:
- ACCGGCGCAAAGTGGTACCTTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MITF (4286)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475375
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4286 | MITF | melanocyte inducing transcr... | NM_001184968.2 | 99.6% | 100% | 270G>A |
| 2 | human | 4286 | MITF | melanocyte inducing transcr... | NM_198158.3 | 21.9% | 21.5% | 262_265delGGAT;271_284delinsG;291_1239del |
| 3 | human | 4286 | MITF | melanocyte inducing transcr... | NM_000248.3 | 21.6% | 21.2% | 262_265delGGAT;271_284delinsG;291_1257del |
| 4 | human | 4286 | MITF | melanocyte inducing transcr... | NM_001184967.2 | 17.8% | 16.9% | (many diffs) |
| 5 | human | 4286 | MITF | melanocyte inducing transcr... | NM_001354608.2 | 17.8% | 16.9% | (many diffs) |
| 6 | human | 4286 | MITF | melanocyte inducing transcr... | NM_001354607.2 | 16.6% | 15.7% | (many diffs) |
| 7 | human | 4286 | MITF | melanocyte inducing transcr... | NM_198177.3 | 16.5% | 15.7% | (many diffs) |
| 8 | human | 4286 | MITF | melanocyte inducing transcr... | NM_001354606.2 | 16% | 15.2% | (many diffs) |
| 9 | human | 4286 | MITF | melanocyte inducing transcr... | NM_006722.2 | 16% | 15.2% | (many diffs) |
| 10 | human | 4286 | MITF | melanocyte inducing transcr... | NM_198159.3 | 16% | 15.2% | (many diffs) |
| 11 | human | 4286 | MITF | melanocyte inducing transcr... | NM_001354605.2 | 15.9% | 15% | (many diffs) |
| 12 | human | 4286 | MITF | melanocyte inducing transcr... | NM_001354604.2 | 15.8% | 15% | (many diffs) |
| 13 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505695.3 | 20.5% | 20.3% | (many diffs) |
| 14 | mouse | 17342 | Mitf | microphthalmia-associated t... | NM_008601.3 | 20.2% | 20% | (many diffs) |
| 15 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505696.1 | 18.3% | 17.6% | (many diffs) |
| 16 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505692.3 | 16.5% | 15.8% | (many diffs) |
| 17 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505693.1 | 16.5% | 15.8% | (many diffs) |
| 18 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505694.1 | 16.5% | 15.8% | (many diffs) |
| 19 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_017321428.1 | 15.8% | 15.1% | (many diffs) |
| 20 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505691.3 | 15.7% | 15.1% | (many diffs) |
| 21 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505689.3 | 15.4% | 14.7% | (many diffs) |
| 22 | mouse | 17342 | Mitf | microphthalmia-associated t... | NM_001178049.1 | 15.4% | 14.7% | (many diffs) |
| 23 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505685.3 | 15.1% | 14.4% | (many diffs) |
| 24 | mouse | 17342 | Mitf | microphthalmia-associated t... | XM_006505684.2 | 14.9% | 14.3% | (many diffs) |
| 25 | mouse | 17342 | Mitf | microphthalmia-associated t... | NM_001113198.1 | 14.9% | 14.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 339
- ORF length:
- 273
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCT GGAAATGCTA GAATATAATC ACTATCAGGT GCAGACCCAC CTCGAAAACC 121 CCACCAAGTA CCACATACAG CAAGCCCAAC GGCAGCAGGT AAAGCAGTAC CTTTCTACCA 181 CTTTAGCAAA TAAACATGCC AACCAAGTCC TGAGCTTGCC ATGTCCAAAC CAGCCTGGCG 241 ATCATGTCAT GCCACCGGTG CCGGGGAGCA GCGCACCCAA CAGCCCCATG GCTATGCTTA 301 CGCTTAACTC CAACTGTGAA AAAGAGTTTA TGAAACAGTG CCCAACTTTC TTGTACAAAG 361 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 421 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 481 ACGAACCGGC GCAAAGTGGT ACCTTATTAC GCGTTAAGTC gacaatcaac ctctggatta 541 caaaatttgt gaaagatt