Transcript: Mouse XM_006507922.2

PREDICTED: Mus musculus family with sequence similarity 168, member A (Fam168a), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fam168a (319604)
Length:
5496
CDS:
273..980

Additional Resources:

NCBI RefSeq record:
XM_006507922.2
NBCI Gene record:
Fam168a (319604)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006507922.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000217449 GATCCAACAAAGTACGTTAAC pLKO.1 4807 3UTR 100% 10.800 15.120 N Fam168a n/a
2 TRCN0000202186 CATCACCCAATCCCTATCAGA pLKO.1 619 CDS 100% 3.000 4.200 N Fam168a n/a
3 TRCN0000192682 GTATCCAATCAGAAGTGCCTA pLKO.1 647 CDS 100% 2.640 3.696 N Fam168a n/a
4 TRCN0000202172 CTTTCAGATATACCGCAGGGA pLKO.1 544 CDS 100% 0.660 0.924 N Fam168a n/a
5 TRCN0000264245 CTCTGCGGCTTTCAGATATAC pLKO_005 536 CDS 100% 13.200 10.560 N FAM168A n/a
6 TRCN0000264244 CCTATCAGACGGCCATGTATC pLKO_005 631 CDS 100% 10.800 8.640 N FAM168A n/a
7 TRCN0000189552 CCTCTGCGGCTTTCAGATATA pLKO.1 535 CDS 100% 13.200 9.240 N Fam168a n/a
8 TRCN0000189924 GAACATGGCCTACACTGGTTA pLKO.1 326 CDS 100% 4.950 3.465 N Fam168a n/a
9 TRCN0000190202 CCTAAGAACATGGCCTACACT pLKO.1 321 CDS 100% 3.000 2.100 N Fam168a n/a
10 TRCN0000201998 CTCTGCTGATGAAACAAGCCT pLKO.1 427 CDS 100% 0.750 0.450 N Fam168a n/a
11 TRCN0000173087 CAGATGAGGAAACTGAGGCTT pLKO.1 2129 3UTR 100% 2.640 1.320 Y FLJ45966 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006507922.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02736 pDONR223 100% 94.8% 100% None (many diffs) n/a
2 ccsbBroad304_02736 pLX_304 0% 94.8% 100% V5 (many diffs) n/a
3 TRCN0000469372 TCTCTTAAGACTTCGACGTGGACC pLX_317 53.7% 94.8% 100% V5 (many diffs) n/a
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