Transcript: Mouse XM_006513539.3

PREDICTED: Mus musculus zinc finger, C3H1-type containing (Zfc3h1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Zfc3h1 (216345)
Length:
6318
CDS:
240..6224

Additional Resources:

NCBI RefSeq record:
XM_006513539.3
NBCI Gene record:
Zfc3h1 (216345)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513539.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000267616 ACCCTCAGAATGCGGAAATTT pLKO_005 5245 CDS 100% 15.000 10.500 N Zfc3h1 n/a
2 TRCN0000267572 GACAGTGCAGGAGGCATATAT pLKO_005 1797 CDS 100% 15.000 10.500 N Zfc3h1 n/a
3 TRCN0000252801 AGATTGGAGTCAGCCTAAATG pLKO_005 6281 3UTR 100% 13.200 9.240 N Zfc3h1 n/a
4 TRCN0000420295 AGATTGGAGTCAGCCTAAATG pLKO_005 6281 3UTR 100% 13.200 9.240 N ZFC3H1 n/a
5 TRCN0000252800 CACCGATAATGCTAGTATTAC pLKO_005 1064 CDS 100% 13.200 9.240 N Zfc3h1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513539.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13369 pDONR223 100% 15.5% 15.1% None (many diffs) n/a
2 ccsbBroad304_13369 pLX_304 0% 15.5% 15.1% V5 (many diffs) n/a
3 TRCN0000473285 TCTCTCACAGCCAAACTCCAGTCA pLX_317 51.5% 15.5% 15.1% V5 (many diffs) n/a
4 ccsbBroadEn_13370 pDONR223 100% 15.1% 15.1% None (many diffs) n/a
5 ccsbBroad304_13370 pLX_304 0% 15.1% 15.1% V5 (many diffs) n/a
6 TRCN0000467153 GCACGACAGCTACGTCGTTTAACC pLX_317 32% 15.1% 15.1% V5 (many diffs) n/a
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