Transcript: Mouse XM_006519293.1

PREDICTED: Mus musculus adaptor-related protein complex 3, mu 1 subunit (Ap3m1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ap3m1 (55946)
Length:
3279
CDS:
224..1480

Additional Resources:

NCBI RefSeq record:
XM_006519293.1
NBCI Gene record:
Ap3m1 (55946)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006519293.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430284 ATGGAAATTTCCGACTCATAT pLKO_005 984 CDS 100% 13.200 18.480 N AP3M1 n/a
2 TRCN0000305487 GGATAATGTGGTCATAGTATA pLKO_005 532 CDS 100% 13.200 18.480 N Ap3m1 n/a
3 TRCN0000305431 TTCTACGTTCTGTCGTCAATT pLKO_005 639 CDS 100% 13.200 18.480 N Ap3m1 n/a
4 TRCN0000421847 GATAATGTGGTCATAGTATAT pLKO_005 533 CDS 100% 13.200 9.240 N AP3M1 n/a
5 TRCN0000111662 GCTTGCTATTTCAGGCTTAAA pLKO.1 1366 CDS 100% 13.200 9.240 N Ap3m1 n/a
6 TRCN0000305486 TGAACCGCTTGGACATGTATG pLKO_005 1389 CDS 100% 10.800 7.560 N Ap3m1 n/a
7 TRCN0000111660 CGGAGCTTTAAGCTAAGGAAA pLKO.1 1633 3UTR 100% 4.950 3.465 N Ap3m1 n/a
8 TRCN0000309923 CGGAGCTTTAAGCTAAGGAAA pLKO_005 1633 3UTR 100% 4.950 3.465 N Ap3m1 n/a
9 TRCN0000111664 CCAGTCAGTGTGTGACTATTT pLKO.1 298 CDS 100% 1.320 0.924 N Ap3m1 n/a
10 TRCN0000309922 CCAGTCAGTGTGTGACTATTT pLKO_005 298 CDS 100% 1.320 0.924 N Ap3m1 n/a
11 TRCN0000348337 TGAAGAGATAGATGCAATTAT pLKO_005 775 CDS 100% 15.000 9.000 N Ap3m1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006519293.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02978 pDONR223 100% 92.9% 99.5% None (many diffs) n/a
2 ccsbBroad304_02978 pLX_304 0% 92.9% 99.5% V5 (many diffs) n/a
3 TRCN0000492244 GCGCCTCCCCGGCGTCCGTGGCCA pLX_317 30.7% 92.9% 99.5% V5 (many diffs) n/a
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