Construct: ORF ccsbBroadEn_04898

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010096.1_s300c1, BRDN0000388336
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PACRG (135138)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 135138 PACRG parkin coregulated NM_001080378.1 100% 100%
2 human 135138 PACRG parkin coregulated NM_001080379.1 100% 100%
3 human 135138 PACRG parkin coregulated NM_152410.2 86.8% 86.8% 613_729del
4 human 135138 PACRG parkin coregulated XM_017010280.2 84.9% 75% (many diffs)
5 human 135138 PACRG parkin coregulated XM_006715344.4 83.3% 80.9% (many diffs)
6 human 135138 PACRG parkin coregulated XM_011535462.3 82.7% 69.7% (many diffs)
7 human 135138 PACRG parkin coregulated XM_011535461.3 82.7% 75% (many diffs)
8 human 135138 PACRG parkin coregulated XM_005266826.5 81.2% 80.2% (many diffs)
9 human 135138 PACRG parkin coregulated XM_005266825.5 81.1% 78.8% (many diffs)
10 human 135138 PACRG parkin coregulated XM_006715345.4 80.2% 80.5% (many diffs)
11 human 135138 PACRG parkin coregulated XM_011535469.3 80.1% 80.1% 612_613insGTGAACTCCGGAGAC;618_619ins138
12 human 135138 PACRG parkin coregulated XM_011535468.3 79.8% 79.3% 614_615delGC;618_622delGGCTCinsAA;627_628ins149
13 human 135138 PACRG parkin coregulated XM_011535466.2 79% 79.3% (many diffs)
14 human 135138 PACRG parkin coregulated XM_017010275.2 71.5% 57.2% (many diffs)
15 human 135138 PACRG parkin coregulated XM_017010276.2 71.5% 57.2% (many diffs)
16 human 135138 PACRG parkin coregulated XM_017010277.2 71.5% 57.2% (many diffs)
17 human 135138 PACRG parkin coregulated XM_017010278.2 71.5% 57.2% (many diffs)
18 human 135138 PACRG parkin coregulated XM_017010279.2 71.5% 57.2% (many diffs)
19 human 135138 PACRG parkin coregulated XM_011535471.2 55.8% 39.2% (many diffs)
20 human 135138 PACRG parkin coregulated XM_017010281.2 36.9% 25.4% (many diffs)
21 human 135138 PACRG parkin coregulated XM_017010282.2 36.9% 25.4% (many diffs)
22 mouse 69310 Pacrg PARK2 co-regulated NM_027032.2 83.2% 86.3% (many diffs)
23 mouse 69310 Pacrg PARK2 co-regulated XM_006523353.3 47.9% 50.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
837
ORF length:
771
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGT GGCAGAAAAA GAGACCCTGA GCTTAAACAA ATGCCCAGAC AAGATGCCGA
121AGAGGACCAA GCTGCTGGCA CAACAGCCGC TCCCGGTGCA CCAGCCTCAC TCTCTGGTTT
181CTGAGGGTTT CACAGTCAAA GCCATGATGA AAAACTCAGT CGTGAGAGGC CCTCCAGCTG
241CAGGGGCATT TAAAGAAAGA CCAACCAAGC CCACAGCATT TCGAAAATTC TATGAGCGAG
301GTGACTTCCC AATTGCCCTT GAGCATGATT CGAAAGGAAA CAAAATCGCC TGGAAGGTAG
361AAATTGAGAA GCTGGATTAC CATCATTATC TGCCTCTGTT TTTTGATGGG CTTTGTGAAA
421TGACATTTCC CTATGAGTTT TTTGCTCGGC AAGGAATCCA CGACATGCTG GAACACGGTG
481GGAACAAGAT CCTACCTGTC CTTCCACAGC TCATTATCCC GATAAAAAAT GCCTTGAACC
541TCCGAAACCG ACAGGTCATC TGTGTCACTC TCAAGGTCCT CCAGCATCTG GTTGTGTCAG
601CTGAGATGGT GGGCAAGGCC TTGGTGCCTT ATTACCGTCA AATCCTCCCT GTCCTGAACA
661TCTTTAAGAA TATGAATGTG AACTCCGGAG ACGGCATTGA CTACAGCCAG CAGAAGAGGG
721AGAACATTGG GGACTTGATC CAGGAGACAC TGGAGGCCTT CGAGCGCTAC GGAGGAGAAA
781ATGCCTTTAT CAACATTAAG TACGTGGTCC CAACCTACGA GTCTTGCTTG CTAAACTACC
841CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
901aac

Download FASTA (ORF) (Full)