Construct: ORF ccsbBroad304_13005
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018370.1_s304c1, BRDN0000408743
- Derived from:
- ccsbBroadEn_13005
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP12 (94134)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13005
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270697.1 | 100% | 100% | |
| 2 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270699.1 | 99.3% | 99.3% | 1230_1231insGCCAGCAGTCCAAAG |
| 3 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | XM_005252644.2 | 99.3% | 99.3% | 1230_1231insGCCAGCAGTCCAAAG |
| 4 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | XM_011519761.2 | 96.6% | 96.6% | 946_947ins81 |
| 5 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270698.2 | 96.2% | 96.2% | 1152_1153ins90 |
| 6 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_018287.7 | 94.4% | 94.4% | 947_1087del |
| 7 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270695.1 | 93.8% | 93.8% | 947_1087del;1371_1372insGCCAGCAGTCCAAAG |
| 8 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | XM_017016954.2 | 92.8% | 92.8% | 946_947ins81;1071_1072ins90 |
| 9 | human | 94134 | ARHGAP12 | Rho GTPase activating prote... | NM_001270696.2 | 90.8% | 90.8% | 947_1087del;1293_1294ins90 |
| 10 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_006526315.1 | 88.5% | 91.2% | (many diffs) |
| 11 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_017318002.1 | 88% | 90.8% | (many diffs) |
| 12 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_006526316.1 | 85.5% | 88.8% | (many diffs) |
| 13 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_006526314.1 | 83.6% | 86.1% | (many diffs) |
| 14 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | NM_001039692.1 | 83.1% | 85.8% | (many diffs) |
| 15 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_011247018.1 | 81.3% | 84.2% | (many diffs) |
| 16 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | NM_029277.2 | 80.8% | 83.9% | (many diffs) |
| 17 | mouse | 75415 | Arhgap12 | Rho GTPase activating prote... | XM_017318001.1 | 79.4% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2466
- ORF length:
- 2397
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaatggct gacagaagtg ggaagattat tccaggacaa gtgtatattg 121 aggtggaata tgattatgaa tatgaagcaa aggacagaaa gattgtgata aaacaagggg 181 agaggtacat cttggtgaaa aagaccaatg atgactggtg gcaagtcaag ccagatgaaa 241 actccaaagc gttttatgtg ccagcccagt atgtgaagga ggtcacgcgc aaagctctca 301 tgccacctgt taagcaggta gctggtctgc caaataactc cacgaaaata atgcagagtt 361 tgcatcttca gagatcaaca gaaaatgtga acaaattgcc tgagctttca agtttcggaa 421 agccatcgtc atctgttcaa ggaacaggtc ttattcgtga tgccaatcag aattttggac 481 ccagttataa tcaaggtcag actgtcaacc taagcctgga cctgacccat aataacggaa 541 agtttaacaa tgactcacat tctcctaaag tttccagcca gaataggaca cgctcatttg 601 gtcattttcc cggtccagag ttcttggatg tagagaaaac tagcttctcc caggaacaat 661 cttgtgattc cgcaggagaa ggctctgaaa gaatacatca agattctgaa tctggtgatg 721 aacttagcag cagctccact gaacagataa gggcaaccac acctccaaat caaggaaggc 781 cagattctcc tgtctatgct aaccttcaag aactgaaaat atcccagtct gctcttcccc 841 cacttcctgg gagcccggca attcagatta atggagaatg ggaaactcat aaagacagct 901 cagggcgttg ctattactat aacagaggga cacaggaaag aacttggaaa cctcctcgtt 961 ggactcggga tgcaagcatc agcaaaggag atttccaaaa tccaggggat caagagtggc 1021 tcaagcatgt tgatgatcaa ggtagacaat attactacag tgcagacgga tctcggtcag 1081 aatgggaatt gccaaagtat aatgcttcat cccagcagca aagagaaata attaaaagta 1141 ggagcctgga caggcggctg caagaaccaa tagtattaac aaagtggaga catagcacca 1201 ttgtattgga cactaatgat aaggaatctc caactgcctc aaaaccctgc tttcctgaaa 1261 atgagtcttc tccctcctca ccaaagcacc aagatacagc cagcagtcca aaggatcaag 1321 agaaatatgg attattaaat gtaacaaaaa ttgctgaaaa tgggaaaaag gttcgaaaga 1381 actggttgtc ttcttgggcg gtgttgcagg gttcatcttt actttttacc aaaactcaag 1441 gaagtagcac aagttggttt ggcagtaatc agtccaaacc agagttcaca gtggacctca 1501 agggggcaac aattgagatg gcttcaaagg ataaatccag caaaaagaat gtatttgagc 1561 tgaaaactcg tcaaggaaca gaactgctaa ttcagtctga caatgacact gttattaatg 1621 attggtttaa agttcttagt agtacaatca ataatcaggc agtagaaact gatgaaggaa 1681 ttgaagagga gataccggat tcaccaggaa tagaaaagca tgataaagaa aaggaacaaa 1741 aggatcccaa aaagcttcgt tcctttaaag tatctagcat agattcttca gaacagaaaa 1801 aaaccaagaa aaacttaaag aagtttctta cacgacgccc cactttgcaa gctgttcgtg 1861 aaaaaggtta tattaaagat caggtatttg gatccaatct cgctaatctg tgtcagagag 1921 agaatggcac agtaccaaag tttgtgaagt tatgtattga acatgttgaa gaacatggtt 1981 tggatattga tgggatatac agagtaagtg gcaacctcgc agtgatccag aaactaaggt 2041 ttgcagtcaa tcatgatgag aaattggact tgaatgacag taaatgggaa gatattcatg 2101 tcattactgg agccctcaaa atgttttttc gagaattacc agaacctctt tttacattta 2161 atcattttaa tgattttgtt aatgcaatta agcaagaacc aagacagcga gtcgctgctg 2221 ttaaggacct aatcagacag ttgccaaagc caaaccaaga cacaatgcag attcttttcc 2281 gacatctcag aagagttata gaaaatggag agaaaaatcg aatgacctat cagagtatag 2341 caattgtttt tggtcccact ctattaaaac cagaaaaaga gactggtaat atagcagttc 2401 atactgtgta ccagaatcag attgtagaat taattcttct ggaactgagt tccatcttcg 2461 gacgtttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2521 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2581 acctctggat ta