Transcript: Human XM_011530042.3

PREDICTED: Homo sapiens peptidylprolyl isomerase like 2 (PPIL2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PPIL2 (23759)
Length:
3192
CDS:
91..1812

Additional Resources:

NCBI RefSeq record:
XM_011530042.3
NBCI Gene record:
PPIL2 (23759)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011530042.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230472 AGGCAGTGGAACAGCTAAATA pLKO_005 476 CDS 100% 15.000 10.500 N PPIL2 n/a
2 TRCN0000230475 TCCACTGAGGGCACGATTTAA pLKO_005 2429 3UTR 100% 15.000 10.500 N PPIL2 n/a
3 TRCN0000230473 AGCTGAACGCTGCCCACTATT pLKO_005 794 CDS 100% 13.200 9.240 N PPIL2 n/a
4 TRCN0000000163 CAACAGCAACAGGTCTCAATT pLKO.1 1209 CDS 100% 13.200 9.240 N PPIL2 n/a
5 TRCN0000230474 ATGTGCTGCGCTACCAGTTTG pLKO_005 896 CDS 100% 10.800 7.560 N PPIL2 n/a
6 TRCN0000219003 CCAATTTGGACAAGTTCAATG pLKO_005 575 CDS 100% 10.800 7.560 N PPIL2 n/a
7 TRCN0000000164 CCAACAGCAACAGGTCTCAAT pLKO.1 1208 CDS 100% 4.950 3.465 N PPIL2 n/a
8 TRCN0000000162 GCTAAATATCAAGGCCAAGAA pLKO.1 489 CDS 100% 4.950 3.465 N PPIL2 n/a
9 TRCN0000000161 TCTCAATTCTTCATCACGTTT pLKO.1 1222 CDS 100% 4.950 3.465 N PPIL2 n/a
10 TRCN0000000160 ACAGCTAAATATCAAGGCCAA pLKO.1 486 CDS 100% 2.160 1.296 N PPIL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011530042.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07929 pDONR223 100% 91.4% 91.9% None (many diffs) n/a
2 ccsbBroad304_07929 pLX_304 0% 91.4% 91.9% V5 (many diffs) n/a
3 TRCN0000469418 ATTAATCTTAGCCAAAAATGCGCA pLX_317 28.2% 91.4% 91.9% V5 (many diffs) n/a
4 ccsbBroadEn_07930 pDONR223 100% 90.2% 85.6% None 1363G>A;1466_1467insGAAG;1557_1719del n/a
5 ccsbBroad304_07930 pLX_304 0% 90.2% 85.6% V5 1363G>A;1466_1467insGAAG;1557_1719del n/a
6 TRCN0000474568 TGAGGTCCAGAGTGGGCCCGGCAC pLX_317 27.2% 90.2% 85.6% V5 1363G>A;1466_1467insGAAG;1557_1719del n/a
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