Transcript: Human XM_011535853.2

PREDICTED: Homo sapiens opioid receptor mu 1 (OPRM1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
OPRM1 (4988)
Length:
1786
CDS:
266..1306

Additional Resources:

NCBI RefSeq record:
XM_011535853.2
NBCI Gene record:
OPRM1 (4988)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011535853.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000357703 TTATGCCAGTGCTCATCATTA pLKO_005 696 CDS 100% 13.200 10.560 N OPRM1 n/a
2 TRCN0000011783 CGGCCAATACAGTGGATAGAA pLKO.1 1098 CDS 100% 5.625 4.500 N OPRM1 n/a
3 TRCN0000368502 TGATCTCCATAGATTACTATA pLKO_005 399 CDS 100% 13.200 9.240 N OPRM1 n/a
4 TRCN0000011784 CCAGAGTGTGAATTACCTAAT pLKO.1 340 CDS 100% 10.800 7.560 N OPRM1 n/a
5 TRCN0000009275 CGTGTGCTATGGACTGATGAT pLKO.1 718 CDS 100% 4.950 3.465 N OPRM1 n/a
6 TRCN0000011785 GCATTGCTCTAGGTTACACAA pLKO.1 933 CDS 100% 4.950 3.465 N OPRM1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011535853.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01122 pDONR223 100% 66.4% 64% None (many diffs) n/a
2 ccsbBroad304_01122 pLX_304 0% 66.4% 64% V5 (many diffs) n/a
3 TRCN0000488117 GCCCGGGTCACCAAGCGACCACCG pLX_317 15.7% 58.3% 56.3% V5 (many diffs) n/a
4 TRCN0000492306 TTGATCTCAAGGTTACTTTAACGC pLX_317 30.6% 58.3% 56.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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