Construct: ORF ccsbBroadEn_01122
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001177.1_s300c1, BRDN0000456321
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OPRM1 (4988)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_000914.4 | 100% | 100% | |
| 2 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145283.2 | 98% | 97.2% | (many diffs) |
| 3 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001008504.3 | 97.5% | 97.2% | (many diffs) |
| 4 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145284.3 | 97.2% | 95.5% | (many diffs) |
| 5 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145285.2 | 97.2% | 97% | 1164_1165insCTAGAAAATCTGGA;1167_1168insAGAAACTGCTCCGTTGCCC |
| 6 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145282.2 | 95.8% | 96% | (many diffs) |
| 7 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001285523.2 | 94.7% | 94.2% | (many diffs) |
| 8 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145286.2 | 94.6% | 94% | (many diffs) |
| 9 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001008503.2 | 93% | 91.5% | (many diffs) |
| 10 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001008505.2 | 88.8% | 86% | (many diffs) |
| 11 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145279.3 | 81.1% | 81.1% | 1_279del |
| 12 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001285524.1 | 81.1% | 81.1% | 1_279del |
| 13 | human | 4988 | OPRM1 | opioid receptor mu 1 | NR_104349.1 | 79% | (many diffs) | |
| 14 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145281.2 | 78% | 77% | (many diffs) |
| 15 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145280.3 | 75% | 75% | 0_1ins300 |
| 16 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001145287.2 | 75% | 75% | 0_1ins300 |
| 17 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001285526.1 | 75% | 75% | 0_1ins300 |
| 18 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_017010906.2 | 75% | 75% | 0_1ins300 |
| 19 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001285527.1 | 72.5% | 72.2% | (many diffs) |
| 20 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001285528.2 | 70.7% | 70.1% | (many diffs) |
| 21 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_011535851.3 | 66.4% | 64% | (many diffs) |
| 22 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_011535853.2 | 66.4% | 64% | (many diffs) |
| 23 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_011535856.2 | 66.4% | 64% | (many diffs) |
| 24 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_011535862.2 | 66.4% | 64% | (many diffs) |
| 25 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_017010903.2 | 66.4% | 64% | (many diffs) |
| 26 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_017010904.1 | 66.4% | 64% | (many diffs) |
| 27 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_017010905.1 | 66.4% | 64% | (many diffs) |
| 28 | human | 4988 | OPRM1 | opioid receptor mu 1 | NR_104351.1 | 44.4% | (many diffs) | |
| 29 | human | 4988 | OPRM1 | opioid receptor mu 1 | XM_017010907.2 | 25.4% | 22% | (many diffs) |
| 30 | human | 4988 | OPRM1 | opioid receptor mu 1 | NM_001285522.1 | 24.1% | 24% | 291_291delCins910 |
| 31 | human | 4988 | OPRM1 | opioid receptor mu 1 | NR_104348.1 | 7.9% | 1_134del;1297_1405del;1444_15079del | |
| 32 | human | 4988 | OPRM1 | opioid receptor mu 1 | NR_104350.1 | 5.6% | 1_134del;422_423ins353;982_14617del | |
| 33 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001302793.1 | 87.2% | 94% | (many diffs) |
| 34 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001304950.1 | 85.6% | 91.5% | (many diffs) |
| 35 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001304937.1 | 85.1% | 91% | (many diffs) |
| 36 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001304938.1 | 84.9% | 91.2% | (many diffs) |
| 37 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | XM_006512437.3 | 84.2% | 90.5% | (many diffs) |
| 38 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001304948.1 | 84% | 87.1% | (many diffs) |
| 39 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | XM_017313827.1 | 80.7% | 84.7% | (many diffs) |
| 40 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001039652.2 | 77.4% | 83.4% | (many diffs) |
| 41 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | XM_017313826.1 | 75.3% | 80.1% | (many diffs) |
| 42 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001304955.1 | 71.6% | 77.1% | (many diffs) |
| 43 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001302794.1 | 71.2% | 71.1% | (many diffs) |
| 44 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001302796.1 | 69.7% | 68.8% | (many diffs) |
| 45 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | NM_001302795.1 | 63% | 63.1% | (many diffs) |
| 46 | mouse | 18390 | Oprm1 | opioid receptor, mu 1 | XM_017313828.1 | 47.9% | 21% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1269
- ORF length:
- 1200
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGACAGCAGC GCTGCCCCCA CGAACGCCAG CAATTGCACT GATGCCTTGG 121 CGTACTCAAG TTGCTCCCCA GCACCCAGCC CCGGTTCCTG GGTCAACTTG TCCCACTTAG 181 ATGGCAACCT GTCCGACCCA TGCGGTCCGA ACCGCACCGA CCTGGGCGGG AGAGACAGCC 241 TGTGCCCTCC GACCGGCAGT CCCTCCATGA TCACGGCCAT CACGATCATG GCCCTCTACT 301 CCATCGTGTG CGTGGTGGGG CTCTTCGGAA ACTTCCTGGT CATGTATGTG ATTGTCAGAT 361 ACACCAAGAT GAAGACTGCC ACCAACATCT ACATTTTCAA CCTTGCTCTG GCAGATGCCT 421 TAGCCACCAG TACCCTGCCC TTCCAGAGTG TGAATTACCT AATGGGAACA TGGCCATTTG 481 GAACCATCCT TTGCAAGATA GTGATCTCCA TAGATTACTA TAACATGTTC ACCAGCATAT 541 TCACCCTCTG CACCATGAGT GTTGATCGAT ACATTGCAGT CTGCCACCCT GTCAAGGCCT 601 TAGATTTCCG TACTCCCCGA AATGCCAAAA TTATCAATGT CTGCAACTGG ATCCTCTCTT 661 CAGCCATTGG TCTTCCTGTA ATGTTCATGG CTACAACAAA ATACAGGCAA GGTTCCATAG 721 ATTGTACACT AACATTCTCT CATCCAACCT GGTACTGGGA AAACCTGCTG AAGATCTGTG 781 TTTTCATCTT CGCCTTCATT ATGCCAGTGC TCATCATTAC CGTGTGCTAT GGACTGATGA 841 TCTTGCGCCT CAAGAGTGTC CGCATGCTCT CTGGCTCCAA AGAAAAGGAC AGGAATCTTC 901 GAAGGATCAC CAGGATGGTG CTGGTGGTGG TGGCTGTGTT CATCGTCTGC TGGACTCCCA 961 TTCACATTTA CGTCATCATT AAAGCCTTGG TTACAATCCC AGAAACTACG TTCCAGACTG 1021 TTTCTTGGCA CTTCTGCATT GCTCTAGGTT ACACAAACAG CTGCCTCAAC CCAGTCCTTT 1081 ATGCATTTCT GGATGAAAAC TTCAAACGAT GCTTCAGAGA GTTCTGTATC CCAACCTCTT 1141 CCAACATTGA GCAACAAAAC TCCACTCGAA TTCGTCAGAA CACTAGAGAC CACCCCTCCA 1201 CGGCCAATAC AGTGGATAGA ACTAATCATC AGCTAGAAAA TCTGGAAGCA GAAACTGCTC 1261 CGTTGCCCTT GCCAACTTTC TTGTACAAAG TTGGcattat aagaaagcat tgcttatcaa 1321 tttgttgcaa cgaac