Transcript: Human XM_011538747.2

PREDICTED: Homo sapiens phosphatidylinositol-5-phosphate 4-kinase type 2 gamma (PIP4K2C), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PIP4K2C (79837)
Length:
867
CDS:
103..852

Additional Resources:

NCBI RefSeq record:
XM_011538747.2
NBCI Gene record:
PIP4K2C (79837)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144940 GGGGATGTACCGAGTCAGTG pXPR_003 TGG 574 77% 5 0.4334 PIP4K2C PIP4K2C 77684
2 BRDN0001149536 GCTTCCTTATCTCCTACGAT pXPR_003 CGG 435 58% 4 -0.0312 PIP4K2C PIP4K2C 77683
3 BRDN0001148338 CATCAATGCCAAATCGATCA pXPR_003 CGG 338 45% 3 -0.1797 PIP4K2C PIP4K2C 77681
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011538747.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000194793 CGTGATCGATTTGGCATTGAT pLKO.1 436 CDS 100% 5.625 7.875 N PIP4K2C n/a
2 TRCN0000199809 GCGTAGCCCACTCGATCAATG pLKO.1 263 CDS 100% 3.600 5.040 N PIP4K2C n/a
3 TRCN0000037720 CGCTTCCTTATCTCCTACGAT pLKO.1 520 CDS 100% 3.000 4.200 N PIP4K2C n/a
4 TRCN0000024702 CTCCAAGATCAAGGTCAACAA pLKO.1 339 CDS 100% 4.950 3.465 N Pip4k2c n/a
5 TRCN0000319779 CTCCAAGATCAAGGTCAACAA pLKO_005 339 CDS 100% 4.950 3.465 N Pip4k2c n/a
6 TRCN0000037719 GCCCAGTCATTTCAAGTTCAA pLKO.1 384 CDS 100% 4.950 3.465 N PIP4K2C n/a
7 TRCN0000289312 GCCCAGTCATTTCAAGTTCAA pLKO_005 384 CDS 100% 4.950 3.465 N PIP4K2C n/a
8 TRCN0000037723 CCTCTCCAACTATCACCAGTA pLKO.1 597 CDS 100% 4.050 2.835 N PIP4K2C n/a
9 TRCN0000199085 CCTCAAGGGTTCCCTAGTGTC pLKO.1 756 CDS 100% 1.350 0.945 N PIP4K2C n/a
10 TRCN0000296192 CCTCAAGGGTTCCCTAGTGTC pLKO_005 756 CDS 100% 1.350 0.945 N PIP4K2C n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011538747.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15154 pDONR223 100% 56.5% 83.8% None (many diffs) n/a
2 ccsbBroad304_15154 pLX_304 0% 56.5% 83.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000466306 CTAATCTCCCGAAAGCCTCATTGA pLX_317 24.6% 56.5% 83.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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