Transcript: Human XM_011544985.1

PREDICTED: Homo sapiens ankyrin repeat domain 13D (ANKRD13D), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANKRD13D (338692)
Length:
2230
CDS:
114..2078

Additional Resources:

NCBI RefSeq record:
XM_011544985.1
NBCI Gene record:
ANKRD13D (338692)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011544985.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000166518 CCGGAACTGCTCAACAAACTT pLKO.1 438 CDS 100% 5.625 7.875 N ANKRD13D n/a
2 TRCN0000164831 CATTCCGGAACTGCTCAACAA pLKO.1 434 CDS 100% 4.950 3.465 N ANKRD13D n/a
3 TRCN0000162051 CGATTTCTACGTTGAGATGAA pLKO.1 470 CDS 100% 4.950 3.465 N ANKRD13D n/a
4 TRCN0000162319 CTGAGAAGATGGAAACTGTTA pLKO.1 874 CDS 100% 4.950 3.465 N ANKRD13D n/a
5 TRCN0000166452 CGTTGAGATGAAGTGGGAGTT pLKO.1 479 CDS 100% 4.050 2.835 N ANKRD13D n/a
6 TRCN0000165930 GACACTCGTAATGTGGCCTTT pLKO.1 819 CDS 100% 4.050 2.835 N ANKRD13D n/a
7 TRCN0000165095 GAAGACTCCATTCCAGTCCTT pLKO.1 998 CDS 100% 2.640 1.848 N ANKRD13D n/a
8 TRCN0000165836 GATGGAAACTGTTAGCGGCTA pLKO.1 881 CDS 100% 2.160 1.512 N ANKRD13D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011544985.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16158 pDONR223 0% 79.1% 79.2% None 1_261del;1072_1218del;1485T>C n/a
2 ccsbBroad304_16158 pLX_304 0% 79.1% 79.2% V5 1_261del;1072_1218del;1485T>C n/a
3 ccsbBroadEn_14474 pDONR223 100% 78.9% 78.7% None (many diffs) n/a
4 ccsbBroad304_14474 pLX_304 0% 78.9% 78.7% V5 (many diffs) n/a
5 TRCN0000476082 TCCGCTAAGCCGGGTGGCATAATT pLX_317 23.2% 78.9% 78.7% V5 (many diffs) n/a
6 ccsbBroadEn_16157 pDONR223 0% 38.4% 38.1% None (many diffs) n/a
7 ccsbBroad304_16157 pLX_304 0% 38.4% 38.1% V5 (many diffs) n/a
8 TRCN0000465531 TGACTCCTCTATTCTTTGGCACAT pLX_317 47.1% 38.4% 38.1% V5 (many diffs) n/a
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