Construct: ORF TRCN0000491369
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019091.2_s317c1
- DNA Barcode:
- GCCTTTCTAGTGGCGGGGCCTAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AK5 (26289)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491369
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26289 | AK5 | adenylate kinase 5 | NM_174858.3 | 99.9% | 99.8% | 1686_1687insG |
| 2 | human | 26289 | AK5 | adenylate kinase 5 | XM_005270739.5 | 95.6% | 95.5% | 246_247ins72;1614_1615insG |
| 3 | human | 26289 | AK5 | adenylate kinase 5 | NM_012093.4 | 95.3% | 95.2% | 0_1ins78;1608_1609insG |
| 4 | human | 26289 | AK5 | adenylate kinase 5 | XM_006710572.3 | 95.3% | 95.2% | 0_1ins78;1608_1609insG |
| 5 | human | 26289 | AK5 | adenylate kinase 5 | XM_017001008.2 | 95.3% | 95.2% | 0_1ins78;1608_1609insG |
| 6 | human | 26289 | AK5 | adenylate kinase 5 | XM_017001009.1 | 95.3% | 95.2% | 0_1ins78;1608_1609insG |
| 7 | human | 26289 | AK5 | adenylate kinase 5 | XM_017001010.2 | 91% | 90.9% | 0_1ins78;168_169ins72;1536_1537insG |
| 8 | human | 26289 | AK5 | adenylate kinase 5 | XM_017001011.2 | 91% | 90.9% | 0_1ins78;168_169ins72;1536_1537insG |
| 9 | human | 26289 | AK5 | adenylate kinase 5 | XM_017001012.1 | 68.8% | 68.5% | (many diffs) |
| 10 | mouse | 229949 | Ak5 | adenylate kinase 5 | NM_001081277.1 | 86.3% | 91.2% | (many diffs) |
| 11 | mouse | 229949 | Ak5 | adenylate kinase 5 | XM_011240115.2 | 84.4% | 87.3% | (many diffs) |
| 12 | mouse | 229949 | Ak5 | adenylate kinase 5 | XM_006501440.3 | 82.9% | 87.9% | (many diffs) |
| 13 | mouse | 229949 | Ak5 | adenylate kinase 5 | XM_006501441.3 | 54.8% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1761
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaacacc aacgatgcca aggagtatct ggcccggagg gaaatccctc 121 agctttttga gagccttttg aatggactga tgtgttctaa gcccgaagat ccagtagaat 181 acttggaaag ttgtttacaa aaagtaaagg aactgggtgg ctgtgacaag gtgaaatggg 241 atacatttgt aagccaggaa aagaagacct tacctccact aaatggagga cagtcacgga 301 gatcctttct aagaaatgta atgcctgaaa actcaaactt tccatatcgg cggtatgacc 361 ggctccctcc aatccatcaa ttctccatag aaagtgacac ggatctctct gagactgcag 421 agttgattga ggagtatgag gtttttgatc ctaccagacc tcgaccaaaa atcattcttg 481 ttataggtgg tccaggaagt ggaaagggta ctcagagttt gaaaattgca gaacgatatg 541 gattccaata catttctgtg ggagaattat taagaaagaa gatccacagt accagcagca 601 ataggaaatg gagtcttatt gccaagataa ttacaactgg agaattggcc ccacaggaaa 661 caacaattac agagataaaa caaaaattga tgcaaatacc tgatgaagag ggcattgtta 721 ttgatggatt tccaagagat gttgcccagg ctctatcttt tgaggaccaa atctgtaccc 781 ccgatttggt ggtattcctg gcttgtgcta atcagagact caaagaaaga ttactgaagc 841 gtgcagaaca gcagggccga ccagacgaca atgtaaaagc tacccaaagg agactaatga 901 acttcaagca gaatgctgct ccattggtta aatacttcca ggaaaagggg ctcatcatga 961 catttgatgc cgaccgcgat gaggatgagg tgttctatga catcagcatg gcagttgaca 1021 acaagttatt tccaaacaaa gaggctgcag caggttcaag tgaccttgat ccttcgatga 1081 tattggacac tggagagatc attgatacag gatctgatta tgaagatcag ggtgatgacc 1141 agttaaatgt atttggagag gacactatgg gaggtttcat ggaagatttg agaaagtgta 1201 aaattatttt cataattggt ggtcctggct ctggcaaagg cacacagtgt gaaaagctgg 1261 tggaaaaata tggatttaca catctctcaa ctggcgagct cctgcgtgag gaactggcat 1321 cagaatctga aagaagcaaa ttgatcagag acattatgga acgtggagac ctggtgccct 1381 caggcatcgt tttggagctc ctgaaggagg ccatggtggc cagcctcggg gacaccaggg 1441 gcttccTGAT TGACGGCTAT CCTCGGGAGG TGAAGCAAGG GGAAGAGTTC GGACGCAGGA 1501 TTGGAGACCC ACAGTTGGTG ATCTGTATGG ACTGCTCGGC AGACACCATG ACCAACCGCC 1561 TTCTCCAAAG GAGCCGGAGC AGCCTGCCTG TGGACGACAC CACCAAGACC ATCGCCAAGC 1621 GCCTAGAAGC CTACTACCGA GCGTCCATCC CCGTGATCGC CTACTACGAG ACAAAAACAC 1681 AGCTACACAA GATAAATGCA GAGGGAACAC CAGAGGACGT TTTTCTTCAA CTCTGCACAG 1741 CTATTGACTC TATTTTCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1801 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1861 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCCTTTC TAGTGGCGGG 1921 GCCTAGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt