Transcript: Human XM_017002174.1

PREDICTED: Homo sapiens small proline rich protein 2B (SPRR2B), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SPRR2B (6701)
Length:
673
CDS:
60..278

Additional Resources:

NCBI RefSeq record:
XM_017002174.1
NBCI Gene record:
SPRR2B (6701)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017002174.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000157177 GAGCAAAGGAACCTTGGCTTT pLKO.1 486 3UTR 100% 4.050 2.835 N SPRR2B n/a
2 TRCN0000157049 GAGACTGAGCAAAGGAACCTT pLKO.1 480 3UTR 100% 3.000 2.100 N SPRR2B n/a
3 TRCN0000157977 CTTTGTCAGTTCCCAGAGCTT pLKO.1 503 3UTR 100% 2.640 1.848 N SPRR2B n/a
4 TRCN0000243252 ATCTTCTCATCAAAGCTTATC pLKO_005 351 3UTR 100% 10.800 6.480 N SPRR2B n/a
5 TRCN0000243251 CACCCTGCCAGCCAAAGTATC pLKO_005 241 CDS 100% 3.600 2.160 N SPRR2B n/a
6 TRCN0000155726 CAGCAGTGCCAGCAGAAATAT pLKO.1 201 CDS 100% 15.000 7.500 Y SPRR2A n/a
7 TRCN0000255630 AGCAGTGCCAGCAGAAATATC pLKO_005 202 CDS 100% 13.200 6.600 Y SPRR2D n/a
8 TRCN0000243248 ATCCACCGAAGAGCAAGTAAC pLKO_005 259 CDS 100% 10.800 5.400 Y SPRR2B n/a
9 TRCN0000152292 CACCTTCATCTTCTCATCAAA pLKO.1 344 3UTR 100% 5.625 2.813 Y SPRR2A n/a
10 TRCN0000152404 CCACCTTCATCTTCTCATCAA pLKO.1 343 3UTR 100% 4.950 2.475 Y SPRR2A n/a
11 TRCN0000154965 GAGCATGAGAGGATAAGGATA pLKO.1 298 3UTR 100% 4.950 2.475 Y SPRR2D n/a
12 TRCN0000243250 GTAACAGCTTCAGGATTCATC pLKO_005 275 CDS 100% 4.950 2.475 Y SPRR2B n/a
13 TRCN0000255628 TAACAGCTTCAGGATTCATCA pLKO_005 276 CDS 100% 4.950 2.475 Y SPRR2D n/a
14 TRCN0000243249 ATATCCTCCTGTGACACCTTC pLKO_005 218 CDS 100% 4.050 2.025 Y SPRR2B n/a
15 TRCN0000154441 GCAGAAATATCCTCCTGTGAC pLKO.1 212 CDS 100% 4.050 2.025 Y SPRR2A n/a
16 TRCN0000156201 CAGTGCCAGCAGAAATATCCT pLKO.1 204 CDS 100% 3.000 1.500 Y SPRR2A n/a
17 TRCN0000155550 CAAAGTATCCACCGAAGAGCA pLKO.1 253 CDS 100% 2.640 1.320 Y SPRR2A n/a
18 TRCN0000152719 CAGAAATATCCTCCTGTGACA pLKO.1 213 CDS 100% 2.640 1.320 Y SPRR2A n/a
19 TRCN0000156140 CAGCAGAAATATCCTCCTGTG pLKO.1 210 CDS 100% 2.250 1.125 Y SPRR2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017002174.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06991 pDONR223 100% 99% 98.6% None 75G>A;193C>T n/a
2 ccsbBroad304_06991 pLX_304 0% 99% 98.6% V5 75G>A;193C>T n/a
3 TRCN0000467715 TCCTGGGTCCGAGCTCTAAATTCC pLX_317 100% 99% 98.6% V5 75G>A;193C>T n/a
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