Transcript: Human XM_017005548.1

PREDICTED: Homo sapiens autophagy related 7 (ATG7), transcript variant X14, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ATG7 (10533)
Length:
2975
CDS:
61..2160

Additional Resources:

NCBI RefSeq record:
XM_017005548.1
NBCI Gene record:
ATG7 (10533)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017005548.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000369085 TCCAAAGTTCTTGATCAATAT pLKO_005 2011 CDS 100% 13.200 10.560 N ATG7 n/a
2 TRCN0000364480 CAAGGACATTAAGGGTTATTA pLKO_005 192 CDS 100% 15.000 10.500 N ATG7 n/a
3 TRCN0000007586 GCTTTGGGATTTGACACATTT pLKO.1 1555 CDS 100% 13.200 9.240 N ATG7 n/a
4 TRCN0000377305 GGCGTGAGACACATCACATTT pLKO_005 1192 CDS 100% 13.200 9.240 N ATG7 n/a
5 TRCN0000364479 TGAGTCATCAGTGGATCTAAA pLKO_005 1047 CDS 100% 13.200 9.240 N ATG7 n/a
6 TRCN0000007587 CCCAGCTATTGGAACACTGTA pLKO.1 303 CDS 100% 4.950 3.465 N ATG7 n/a
7 TRCN0000007585 CCAGAGAGTTTACCTCTCATT pLKO.1 526 CDS 100% 0.495 0.347 N ATG7 n/a
8 TRCN0000364478 CTTGACATTTGCAGATCTAAA pLKO_005 459 CDS 100% 13.200 7.920 N ATG7 n/a
9 TRCN0000007588 CCAAGGTCAAAGGACGAAGAT pLKO.1 720 CDS 100% 4.950 2.970 N ATG7 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2205 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017005548.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02460 pDONR223 100% 95.1% 94.8% None (many diffs) n/a
2 ccsbBroad304_02460 pLX_304 0% 95.1% 94.8% V5 (many diffs) n/a
3 TRCN0000472530 ACCACTTTTGACACCTGTACTAAC pLX_317 21.4% 95.1% 94.8% V5 (many diffs) n/a
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