Transcript: Human XM_017011797.1

PREDICTED: Homo sapiens zinc finger protein 679 (ZNF679), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF679 (168417)
Length:
1528
CDS:
231..1466

Additional Resources:

NCBI RefSeq record:
XM_017011797.1
NBCI Gene record:
ZNF679 (168417)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017011797.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412547 TCCGCCAGAGCTAGGCATAAA pLKO_005 521 CDS 100% 13.200 18.480 N ZNF679 n/a
2 TRCN0000020187 CAACTACATCAACATCAGATA pLKO.1 822 CDS 100% 4.950 3.465 N ZNF679 n/a
3 TRCN0000020186 TGTGTCAAAGTCTTCGGCAAA pLKO.1 711 CDS 100% 4.050 2.835 N ZNF679 n/a
4 TRCN0000425185 GCCGCTCCTCAACACTTGCTA pLKO_005 1063 CDS 100% 1.000 0.700 N ZNF679 n/a
5 TRCN0000020188 GATCACGCTCAGCAGAATTTA pLKO.1 330 CDS 100% 15.000 9.000 N ZNF679 n/a
6 TRCN0000436040 GTAACCAAACACCCAGTTATG pLKO_005 477 CDS 100% 10.800 6.480 N ZNF679 n/a
7 TRCN0000020184 CTGCTCTTCAACCCTTTCTAA pLKO.1 896 CDS 100% 5.625 3.375 N ZNF679 n/a
8 TRCN0000020185 CCTCAACTCTTAATACTCATA pLKO.1 1321 CDS 100% 4.950 2.970 N ZNF679 n/a
9 TRCN0000432443 GACATTCAGAGATGTAGTCAT pLKO_005 278 CDS 100% 4.950 2.970 N ZNF679 n/a
10 TRCN0000147126 CCCAGTTATGTGTTCTCATTT pLKO.1 488 CDS 100% 13.200 6.600 Y ZNF43 n/a
11 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1270 CDS 100% 13.200 6.600 Y Zfp934 n/a
12 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1270 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
13 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1270 CDS 100% 13.200 6.600 Y EG668616 n/a
14 TRCN0000016587 CCTGGGTATTGCTGTCTCTAA pLKO.1 392 CDS 100% 4.950 2.475 Y ZNF675 n/a
15 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 362 CDS 100% 4.950 2.475 Y ZNF493 n/a
16 TRCN0000018036 CCCAGTTATGTGTTCTCATAT pLKO.1 488 CDS 100% 13.200 6.600 Y ZNF257 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017011797.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15279 pDONR223 63.5% 99.3% 58.8% None (many diffs) n/a
2 ccsbBroadEn_11384 pDONR223 100% 18.9% 17.7% None (many diffs) n/a
3 ccsbBroad304_11384 pLX_304 0% 18.9% 17.7% V5 (many diffs) n/a
4 TRCN0000470576 TACATACAGACCTACACGTAGACC pLX_317 100% 18.9% 17.7% V5 (many diffs) n/a
5 ccsbBroadEn_13746 pDONR223 100% 16.2% 14.8% None (many diffs) n/a
6 ccsbBroad304_13746 pLX_304 0% 16.2% 14.8% V5 (many diffs) n/a
7 TRCN0000475669 ATTCGCAGCGAGTTTGTCCGCCGC pLX_317 100% 16.2% 14.8% V5 (many diffs) n/a
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