Transcript: Human XM_017011996.2

PREDICTED: Homo sapiens amphiphysin (AMPH), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AMPH (273)
Length:
4036
CDS:
147..2912

Additional Resources:

NCBI RefSeq record:
XM_017011996.2
NBCI Gene record:
AMPH (273)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017011996.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000379836 TCATTCCTTCGGTGGTCATAG pLKO_005 2440 CDS 100% 10.800 15.120 N AMPH n/a
2 TRCN0000008010 CTGATGAACTTACCTTACAAA pLKO.1 2737 CDS 100% 5.625 7.875 N AMPH n/a
3 TRCN0000380812 GCGAGAACTCCGAGGATATTT pLKO_005 317 CDS 100% 15.000 10.500 N AMPH n/a
4 TRCN0000381764 GGGCAATTTCCTGACATAAAG pLKO_005 522 CDS 100% 13.200 9.240 N AMPH n/a
5 TRCN0000380462 AGTCGCTGCATGAAGTCTATG pLKO_005 382 CDS 100% 10.800 7.560 N AMPH n/a
6 TRCN0000380290 GAGACCTTGCCACCTACAAAG pLKO_005 2854 CDS 100% 10.800 7.560 N AMPH n/a
7 TRCN0000008008 CGACAGCAAGTCCAAATCATA pLKO.1 964 CDS 100% 5.625 3.938 N AMPH n/a
8 TRCN0000008007 CCAGAGTATGATCTGCAACTT pLKO.1 2201 CDS 100% 4.950 3.465 N AMPH n/a
9 TRCN0000011220 GCTCTTATTCTGTCTGGCATT pLKO.1 3553 3UTR 100% 4.050 2.835 N AMPH n/a
10 TRCN0000380008 GAAAGTGTTTGAAGAGTTTAA pLKO_005 677 CDS 100% 13.200 7.920 N AMPH n/a
11 TRCN0000008009 CGAATCTCTAAGGCAGAAGAA pLKO.1 639 CDS 100% 4.950 2.970 N AMPH n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017011996.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05811 pDONR223 100% 73.7% 67.3% None (many diffs) n/a
2 ccsbBroad304_05811 pLX_304 0% 73.7% 67.3% V5 (many diffs) n/a
3 TRCN0000476974 ACACGTGTGAATGGGACAGACCTT pLX_317 14.3% 73.7% 67.3% V5 (many diffs) n/a
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