Construct: ORF TRCN0000476974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006495.1_s317c1
- Derived from:
- ccsbBroadEn_05811
- DNA Barcode:
- ACACGTGTGAATGGGACAGACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMPH (273)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 273 | AMPH | amphiphysin | NM_001635.4 | 99.9% | 99.8% | 1487A>C |
| 2 | human | 273 | AMPH | amphiphysin | NM_139316.2 | 93.9% | 93.8% | 1270_1271ins126;1361A>C |
| 3 | human | 273 | AMPH | amphiphysin | XM_006715690.4 | 82.2% | 75.4% | (many diffs) |
| 4 | human | 273 | AMPH | amphiphysin | XM_011515271.3 | 80.6% | 80.3% | (many diffs) |
| 5 | human | 273 | AMPH | amphiphysin | XM_017011996.2 | 73.7% | 67.3% | (many diffs) |
| 6 | human | 273 | AMPH | amphiphysin | XM_006715691.4 | 56% | 55.5% | (many diffs) |
| 7 | human | 273 | AMPH | amphiphysin | XM_006715689.4 | 55.9% | 51.3% | (many diffs) |
| 8 | human | 273 | AMPH | amphiphysin | XM_017011995.2 | 54.8% | 54.6% | (many diffs) |
| 9 | mouse | 218038 | Amph | amphiphysin | NM_001289546.1 | 83% | 85% | (many diffs) |
| 10 | mouse | 218038 | Amph | amphiphysin | NM_175007.2 | 82.7% | 84.9% | (many diffs) |
| 11 | mouse | 218038 | Amph | amphiphysin | XM_011244304.2 | 79.2% | 81.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2151
- ORF length:
- 2085
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgacatcaag acgggcatct tcgccaagaa cgtccagaag cgactcaacc 121 gcgcgcagga aaaggtcctc caaaagctgg ggaaagctga tgagacaaaa gacgaacagt 181 tcgaagaata tgtccagaac ttcaaacggc aagaagcaga gggtaccaga cttcagcgag 241 aactccgagg atatttagca gcaatcaaag gcatgcagga ggcctccatg aagctcacag 301 agtcgctgca tgaagtctat gagcctgact ggtatgggcg ggaagatgtg aaaatggttg 361 gtgagaaatg tgatgtgctg tgggaagact tccatcaaaa actcgtggat gggtccttgc 421 taacactgga tacctacctg gggcaatttc ctgacataaa gaatcgcatc gccaagcgca 481 gcaggaagct agtggactat gacagtgccc gccaccatct ggaagctctg cagagctcca 541 agaggaagga tgagagtcga atctctaagg cagaagaaga atttcagaaa gcacagaaag 601 tgtttgaaga gtttaacgtt gacttacaag aagagttacc atcattatgg tcaagacgag 661 ttggatttta tgttaatact ttcaaaaacg tctccagcct tgaagccaag tttcataagg 721 aaattgcggt gctttgccac aaactgtatg aagtgatgac aaaactgggt gaccagcacg 781 ccgacaaggc cttcaccatc caaggagcgc ccagtgattc gggtcctctc cgcattgcaa 841 agacaccatc accgcctgag gagccttcac ccctcccgag cccgacagca agtccaaatc 901 atacattagc acctgcgtct cccgcaccag cacggcctcg gtcaccttca cagacaagga 961 aagggcctcc tgtcccacct ctacctaaag tcaccccgac aaaggaactg cagcaggaga 1021 acatcatcag tttctttgag gacaactttg ttccagaaat cagtgtgaca acaccttccc 1081 agaatgaagt ccctgaggtg aagaaagagg agactttgct ggatctggac tttgatcctt 1141 tcaagcccga ggtgacacct gcaggttctg ctggagtgac ccactcaccc atgtctcaga 1201 cattgccctg ggacctatgg acgacaagca ctgatttggt acagccggct tctggtggtt 1261 catttaatgg attcacacag ccccaggata cttcattatt cacaatgcag acagaccaga 1321 gtatgatctg caacttggct gaatctgaac aggctccacc cacagagcca aaagcagagg 1381 agcctctggc tgctgtcaca cctgccgttg gtctggacct tggaatggac actcgggctg 1441 aggagccagt ggaggaggca gtgatcatac ctggagctga tgctgatgca gctgttggaa 1501 ccttggtgtc agcagctgag ggggccccag gagaggaagc agaggcggag acggccactg 1561 tccctgccgg ggaaggagta agtttagagg aggccaaaat tggaactgaa accactgagg 1621 gtgcagagag tgcccaacct gaagcagagg agctcgaagc aacagtgcct caggagaagg 1681 tcattccttc ggtggtcata gagcctgcct ccaaccatga agaggaagga gaaaacgaaa 1741 taactatagg tgcagagccc aaggagacca ccgaggacgc ggctcctccg ggccccacca 1801 gcgagacacc ggagctggct acggagcaga agcctatcca ggaccctcag cccacgccTT 1861 CTGCACCAGC CATGGGGGCT GCTGACCAGC TAGCATCTGC AAGGGAGGCC TCTCAGGAAT 1921 TGCCTCCTGG CTTTCTCTAC AAGGTGGAAA CACTGCATGA TTTTGAGGCA GCAAATTCTG 1981 ATGAACTTAC CTTACAAAGG GGTGATGTGG TGCTGGTGGT CCCCTCAGAT TCAGAAGCTG 2041 ATCAGGATGC AGGCTGGCTG GTGGGAGTGA AGGAATCAGA CTGGCTTCAG TACAGAGACC 2101 TTGCCACCTA CAAAGGCCTC TTTCCAGAGA ACTTCACCCG ACGCTTAGAT TACCCAACTT 2161 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2221 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2281 CTTGTGGAAA GGACGAACAC GTGTGAATGG GACAGACCTT ACGCGTTAAG TCgacaatca 2341 acctctggat tacaaaattt gtgaaagatt