Construct: ORF ccsbBroad304_08879
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005662.1_s304c1, BRDN0000462146
- Derived from:
- ccsbBroadEn_08879
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASD1 (64921)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08879
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64921 | CASD1 | CAS1 domain containing 1 | NM_022900.5 | 99.8% | 99.8% | 1156C>A;1464T>C;1857T>C |
| 2 | human | 64921 | CASD1 | CAS1 domain containing 1 | XM_011516495.2 | 90.1% | 88.8% | (many diffs) |
| 3 | human | 64921 | CASD1 | CAS1 domain containing 1 | NM_001363426.1 | 81.9% | 81.9% | (many diffs) |
| 4 | human | 64921 | CASD1 | CAS1 domain containing 1 | NM_001363428.1 | 76.6% | 76.6% | (many diffs) |
| 5 | human | 64921 | CASD1 | CAS1 domain containing 1 | NM_001363427.1 | 65.2% | 64.6% | (many diffs) |
| 6 | human | 64921 | CASD1 | CAS1 domain containing 1 | XM_017012546.2 | 65.2% | 64.6% | (many diffs) |
| 7 | human | 64921 | CASD1 | CAS1 domain containing 1 | XM_006716093.3 | 58.2% | 57.2% | (many diffs) |
| 8 | mouse | 213819 | Casd1 | CAS1 domain containing 1 | NM_145398.2 | 89.6% | 94.7% | (many diffs) |
| 9 | mouse | 213819 | Casd1 | CAS1 domain containing 1 | XM_006505029.2 | 73.3% | 77.3% | (many diffs) |
| 10 | mouse | 213819 | Casd1 | CAS1 domain containing 1 | XM_006505030.2 | 64.4% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2457
- ORF length:
- 2391
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctctggcc tacaacctgg gcaagcggga gatcaaccac tacttcagcg 121 tgaggagcgc caaggtgctg gcgctggtgg ccgtgctgct gctcgcagcg tgccacctcg 181 cctcccgccg ctaccgaggc aatgattcgt gtgaatacct tctctcaagt ggcagatttc 241 ttggagagaa agtttggcaa cctcacagtt gtatgatgca taaatacaaa atcagtgaag 301 caaagaactg ccttgtagat aaacatattg catttattgg agattccaga attcgtcaat 361 tgttttattc ttttgtaaaa ataattaatc cccaattcaa agaagaagga aataagcatg 421 aaaacattcc ttttgaagac aagactgcat cagttaaagt ggattttctg tggcatcctg 481 aagttaatgg ttctatgaaa cagtgtatca aagtgtggac tgaggattcc attgcaaagc 541 cacatgtgat tgtagcagga gctgccacat ggtccatcaa gattcacaat ggtagcagtg 601 aagcgctttc tcaatataaa atgaacatca cctccatagc accactttta gaaaaattgg 661 caaagactag tgatgtttat tgggtcttac aagatcctgt ttatgaagat ctattaagtg 721 aaaataggaa gatgatcact aatgagaaga tagatgctta caatgaagct gcagtcagta 781 ttttgaatag tagcaccaga aattctaaat caaatgttaa gatgttcagt gtttccaaat 841 taattgctca agaaaccatc atggaatctt tggatggctt acatcttcct gaatcgagca 901 gagaaactac tgcaatgatt cttatgaatg tgtattgcaa taagattttg aagcctgtag 961 atgggtcctg ttgtcaacct cggcctcctg ttactctcat acagaagcta gctgcttgtt 1021 ttttcacttt atctattatc ggatatttaa ttttttacat aattcatcgt aatgctcatc 1081 ggaagaataa gccgtgtact gatttggaaa gtggagagga aaagaaaaat attatcaata 1141 cccctgtgtc ttcattagaa atacttttac aatctttctg caaacttggc ctgattatgg 1201 catatttcta tatgtgtgac agtgcaaatc tgttcatgaa ggaaaacaaa ttttatacac 1261 attcatcttt ctttattcca attatctaca ttttggtttt gggagtattt tataatgaaa 1321 atactaaaga gactaaagta ttaaatagag aacaaacaga cgaatggaaa ggctggatgc 1381 aacttgtgat tttgatttat cacatttctg gagcaagtac atttttgcct gtatacatgc 1441 acattcgagt tctggttgct gcatatttat ttcagacagg gtatgggcat ttctcatact 1501 tttggataaa aggagatttt ggaatctaca gagtatgtca ggttttattt cgtctcaatt 1561 tcctggtagt ggtgttatgt atagtaatgg atcgacctta tcaattctat tactttgtcc 1621 ccttggtcac tgtatggttc atggtcatat atgttacttt agcactatgg ccacaaataa 1681 tccaaaaaaa agcaaacgga aattgtttct ggcattttgg cttactgttg aaactaggct 1741 ttttgctgtt attcatatgt tttttggcat attctcaggg tgcatttgag aagatctttt 1801 ctctttggcc attgtccaag tgttttgaac tgaaagggaa tgtatatgaa tggtggttca 1861 gatggaggtt agaccgttat gtagttttcc acggaatgct gtttgctttt atttatctgg 1921 ccttgcagaa gcgtcaaata ctttctgaag gaaagggtga acctcttttt tcaaacaaaa 1981 tttcaaattt tctgttgttt atttcagtag tttctttctt gacctattcc atctgggcta 2041 gcagttgtaa aaacaaagca gagtgcaatg aactccatcc gtctgtttct gtggtacaga 2101 ttttagcctt catcctaata agaaacatcc ctggatatgc ccgttcagtt tacagttcat 2161 tttttgcttg gtttggaaaa atttcattag agctatttat ttgccagtat cacatatggc 2221 tggcagcgga cacaaggggt atcttggtac tgatacctgg aaaccctatg ctcaacatca 2281 ttgtcagcac tttcatattt gtttgtgtgg cacatgaaat ttctcagatc actaatgatc 2341 ttgcacagat tattattcct aaagataact catctctctt gaaaaggttg gcatgtatag 2401 ctgcattttt ttgtggactc ctcatcttat catccattca agataaatca aaacattacc 2461 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2521 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2581 tta