Construct: ORF ccsbBroad304_06606
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015459.1_s304c1, BRDN0000401497
- Derived from:
- ccsbBroadEn_06606
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MUSK (4593)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06606
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4593 | MUSK | muscle associated receptor ... | NM_001166280.2 | 99.9% | 100% | 402G>A |
| 2 | human | 4593 | MUSK | muscle associated receptor ... | XM_017014734.1 | 98.9% | 98.8% | 402G>A;1127_1150del |
| 3 | human | 4593 | MUSK | muscle associated receptor ... | NM_001166281.2 | 98.6% | 98.7% | 402G>A;627_628ins30 |
| 4 | human | 4593 | MUSK | muscle associated receptor ... | XM_005251995.3 | 89.8% | 89.8% | 402G>A;949_1212del |
| 5 | human | 4593 | MUSK | muscle associated receptor ... | XM_005251994.3 | 89% | 88.9% | 402G>A;949_1212del;1391_1414del |
| 6 | human | 4593 | MUSK | muscle associated receptor ... | XM_005251996.3 | 88.7% | 88.7% | 402G>A;627_628ins30;919_1182del |
| 7 | human | 4593 | MUSK | muscle associated receptor ... | NM_005592.4 | 87.9% | 87.8% | (many diffs) |
| 8 | human | 4593 | MUSK | muscle associated receptor ... | NM_001369398.1 | 57.3% | 57.3% | 0_1ins1002 |
| 9 | human | 4593 | MUSK | muscle associated receptor ... | XM_011518708.2 | 56.7% | 56.6% | 0_1ins1002;125_148del |
| 10 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001037129.1 | 79.1% | 84.3% | (many diffs) |
| 11 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001037128.1 | 78.3% | 83.5% | (many diffs) |
| 12 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001037130.1 | 78.2% | 83.5% | (many diffs) |
| 13 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | XM_006537661.2 | 78.2% | 83.4% | (many diffs) |
| 14 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_010944.2 | 77.5% | 82.7% | (many diffs) |
| 15 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | XM_006537659.2 | 77.5% | 82.5% | (many diffs) |
| 16 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001037127.2 | 77% | 82.1% | (many diffs) |
| 17 | mouse | 18198 | Musk | muscle, skeletal, receptor ... | NM_001165996.1 | 38.3% | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2418
- ORF length:
- 2349
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagagagctc gtcaacattc cactggtaca tattcttact ctggttgcct 121 tcagcggaac tgagaaactt ccaaaagctc ctgtcatcac cactcctctt gaaacagtgg 181 atgccttagt tgaagaagtg gctactttca tgtgtgcagt ggaatcctac ccccagcctg 241 agatttcctg gactagaaat aaaattctca ttaaactctt tgacacccgg tacagcatcc 301 gggagaatgg gcagctcctc accatcctga gtgtggaaga cagtgatgat ggcatttact 361 gctgcacggc caacaatggt gtgggaggag ctgtggagag ttgtggagcc ctgcaagtga 421 agatgaaacc taaaataact cgtcctccca taaatgtgaa aataatagaa ggattaaaag 481 cagtcctacc atgtactaca atgggtaatc ccaaaccatc agtgtcttgg ataaagggag 541 acagccctct cagggaaaat tcccgaattg cagttcttga atctgggagc ttgaggattc 601 ataacgtaca aaaggaagat gcaggacagt atcgatgtgt ggcaaaaaac agcctcggga 661 cagcatattc caaagtggtg aagctggaag ttgaggaaga aagtgaaccc gaacaagata 721 ctaaagtttt tgccaggatc ctgcgggctc ctgaatccca caatgtcacc tttggctcct 781 ttgtgaccct gcactgtaca gcaacaggca ttcctgtccc caccatcacc tggattgaaa 841 acggaaatgc tgtttcttct gggtccattc aagagagtgt gaaagaccga gtgattgact 901 caagactgca gctgtttatc accaagccag gactctacac atgcatagct accaataagc 961 atggggagaa gttcagtact gccaaggctg cagccaccat cagcatagca gaatggagag 1021 agtactgctt ggcagtaaag gagctcttct gcgcaaaaga atggctggta atggaagaga 1081 agacccacag aggactctac agatccgaga tgcatttgct gtccgtgcca gaatgcagca 1141 agcttcccag catgcattgg gaccccacgg cctgtgccag actgccacat ctagcattcc 1201 caccaatgac gtcctcaaag ccaagtgtgg acattccaaa tctgccttcc tcctcctctt 1261 cttccttctc tgtctcacct acatactcca tgactgtaat aatctccatc atgtccagct 1321 ttgcaatatt tgtgcttctt accataacta ctctctattg ctgccgaaga agaaaacaat 1381 ggaaaaataa gaaaagagaa tcagcagcag taaccctcac cacactgcct tctgagctct 1441 tactagatag acttcatccc aaccccatgt accagaggat gccgctcctt ctgaacccca 1501 aattgctcag cctggagtat ccaaggaata acattgaata tgtgagagac atcggagagg 1561 gagcgtttgg aagggtgttt caagcaaggg caccaggctt acttccctat gaacctttca 1621 ctatggtggc agtaaagatg ctcaaagaag aagcctcggc agatatgcaa gcggactttc 1681 agagggaggc agccctcatg gcagaatttg acaaccctaa cattgtgaag ctattaggag 1741 tgtgtgctgt cgggaagcca atgtgcctgc tctttgaata catggcctat ggtgacctca 1801 atgagttcct ccgcagcatg tcccctcaca ccgtgtgcag cctcagtcac agtgacttgt 1861 ctatgagggc tcaggtctcc agccctgggc ccccacccct ctcctgtgct gagcagcttt 1921 gcattgccag gcaggtggca gctggcatgg cttacctctc agaacgtaag tttgttcacc 1981 gagatttagc caccaggaac tgcctggtgg gcgagaacat ggtggtgaaa attgccgact 2041 ttggcctctc caggaacatc tactcagcag actactacaa agctaatgaa aacgacgcta 2101 tccctatccg ttggatgcca ccagagtcca ttttttataa ccgctacact acagagtctg 2161 atgtgtgggc ctatggcgtg gtcctctggg agatcttctc ctatggcctg cagccctact 2221 atgggatggc ccatgaggag gtcatttact acgtgcgaga tggcaacatc ctctcctgcc 2281 ctgagaactg ccccgtggag ctgtacaatc tcatgcgtct atgttggagc aagctgcctg 2341 cagacagacc cagtttcacc agtattcacc gaattctgga acgcatgtgt gagagggcag 2401 agggaactgt gagtgtcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 2461 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2521 agtcgacaat caacctctgg atta