Transcript: Human XM_017027735.1

PREDICTED: Homo sapiens ninein like (NINL), transcript variant X15, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NINL (22981)
Length:
3741
CDS:
498..2987

Additional Resources:

NCBI RefSeq record:
XM_017027735.1
NBCI Gene record:
NINL (22981)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017027735.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000369405 ATTAGCGGTCCTGAGTAATTT pLKO_005 3202 3UTR 100% 15.000 21.000 N NINL n/a
2 TRCN0000054338 CCAAGGTAAATTACTACGAAA pLKO.1 550 CDS 100% 4.950 6.930 N NINL n/a
3 TRCN0000054341 GCATCGTGTGACCATTCAGAT pLKO.1 2354 CDS 100% 4.950 6.930 N NINL n/a
4 TRCN0000364671 CAGAACTACAAGGATCAATTA pLKO_005 2265 CDS 100% 13.200 10.560 N NINL n/a
5 TRCN0000376491 TGCTTTAAGCTTAGCTTATTA pLKO_005 3398 3UTR 100% 15.000 10.500 N NINL n/a
6 TRCN0000364595 CATTTCATTCCTGGGTAATTC pLKO_005 449 5UTR 100% 13.200 9.240 N NINL n/a
7 TRCN0000054342 CCTCCAAGAGAAAGTGGATAA pLKO.1 2762 CDS 100% 10.800 7.560 N NINL n/a
8 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4 5UTR 100% 5.625 2.813 Y KLHL30 n/a
9 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017027735.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13803 pDONR223 100% 8.3% 7.1% None (many diffs) n/a
2 ccsbBroad304_13803 pLX_304 0% 8.3% 7.1% V5 (many diffs) n/a
3 TRCN0000471684 AGCCTAGGTCAAATATTTTAATCG pLX_317 100% 8.3% 7.1% V5 (many diffs) n/a
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